PXD055514

PXD055514 is an original dataset announced via ProteomeXchange.

Title	Whole cell iTRAQ proteome analysis of strain JA2
Description	Here, we employed L-tryptophan metabolomics and iTRAQ based proteomic profiling of strain JA2 to decipher the molecular response. Wherein, L-tryptophan catabolism and molecular response of strain JA2 unexpected under chemotrophic conditions. Molecular response of L-tryptophan is essential to our understanding of L-tryptophan catabolism and can be demonstrated the physiological condition of microorganisms, when organisms was exposed with aromatic compounds ever changing the environment conditions. Cellular response such as membrane protein, RND and ABC pumps implicate to efflux of aromatic compounds metabolism of tryptophan by strain JA2. Furthermore, strain JA2 feed with tryptophan 210 differentially regulated proteins related signaling, transcription couple translation, stress, membrane transport and aromatic compounds metabolism highly upregulated. L-tryptophan metabolomics and global proteomic approaches rewiring the amino acid, fatty acid, lipid and energy of cells fed with tryptophan. Aromatic amino acid metabolism proteins upregulated and sulpher containing amino acid were down regulated leading toward the adaptation of L-tryptophan feed by strain JA2.
HostingRepository	PRIDE
AnnounceDate	2024-11-27
AnnouncementXML	Submission_2024-11-26_22:02:30.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Md Shabbir Ahmad
SpeciesList	scientific name: Rubrivivax benzoatilyticus JA2 = ATCC BAA-35; NCBI TaxID: 987059;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Dionex instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-03 05:47:02	ID requested
⏵ 1	2024-11-26 22:02:30	announced

10.1039/D4MO00170B;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: iTRAQ,Anoxygenic photosynthetic bacteria, L-tryptophan metabolism

Prof. CH VENNKATA RAMANA LAB
contact affiliation	UNIVERSITY OF HYDERABAD INDIA 50046
contact email	shabbirmd@instem.res.in
lab head
Md Shabbir Ahmad
contact affiliation	University of Hyderabad
contact email	shabbirmd@instem.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD055514

PRIDE project URI

• PRIDE
  1. PXD055514
     1. Label: PRIDE project
     2. Name: Whole cell iTRAQ proteome analysis of strain JA2