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PXD055501

PXD055501 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImpact of oxygen availability on the organelle-specific redox potentials and stress in recombinant protein producing Komagataella phaffii
DescriptionThe methylotrophic yeast Komagataella phaffii (syn. Pichia pastoris) is one of the most effective and established expression hosts for heterologous protein production. The redox balance of its secretory pathway, which is multi-organelle dependent, is of high importance for recombinant protein production. Redox imbalance and oxidative stress are two main factors that can influence protein production and secretion, especially the redox potential of the ER where protein folding and disulfide bond formation occur. Glutathione is the main redox buffer of the cell and its redox conditions can be determined by the status of the glutathione redox couple (GSH-GSSG). In this study, in vivo measurements of the glutathione redox potential in different subcellular compartments were achieved by genetically encoded redox sensitive fluorescent probes (roGFPs). Using these biosensors, the impact of oxygen availability on the redox potentials of different organelles (cytosol, ER, mitochondria and peroxisomes) of non-producing and producing K. phaffii strains in glucose-limited chemostat cultures was investigated. It was found that the transition from normoxic to hypoxic conditions affected the redox potentials of all investigated organelles. Also, as reported previously, hypoxic conditions led to increased recombinant protein secretion. Finally, transcriptome and proteome datasets analysis provided novel insights into the short-term adaptation of the cells from normoxic to hypoxic conditions. In conclusion, improved production of therapeutic disulfide-bonded proteins in industrial settings can be achieved by tuning oxidative stress and redox homeostasis in the K. phaffii strains.
HostingRepositoryPRIDE
AnnounceDate2025-01-30
AnnouncementXMLSubmission_2025-01-30_02:37:10.188.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandra Maass
SpeciesList scientific name: Komagataella phaffii; NCBI TaxID: 460519;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-03 00:45:28ID requested
12025-01-30 02:37:10announced
Publication List
10.1111/1751-7915.70106;
Keyword List
submitter keyword: Komagataella phaffii, yeast, hypoxia, glutathione redox potential, chemostat cultivation
Contact List
Dörte Becher
contact affiliationDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliationUniversity of Greifswald, Department for Microbial Proteomics
contact emailsandra.maass@uni-greifswald.de
dataset submitter
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