PXD055501 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of oxygen availability on the organelle-specific redox potentials and stress in recombinant protein producing Komagataella phaffii |
Description | The methylotrophic yeast Komagataella phaffii (syn. Pichia pastoris) is one of the most effective and established expression hosts for heterologous protein production. The redox balance of its secretory pathway, which is multi-organelle dependent, is of high importance for recombinant protein production. Redox imbalance and oxidative stress are two main factors that can influence protein production and secretion, especially the redox potential of the ER where protein folding and disulfide bond formation occur. Glutathione is the main redox buffer of the cell and its redox conditions can be determined by the status of the glutathione redox couple (GSH-GSSG). In this study, in vivo measurements of the glutathione redox potential in different subcellular compartments were achieved by genetically encoded redox sensitive fluorescent probes (roGFPs). Using these biosensors, the impact of oxygen availability on the redox potentials of different organelles (cytosol, ER, mitochondria and peroxisomes) of non-producing and producing K. phaffii strains in glucose-limited chemostat cultures was investigated. It was found that the transition from normoxic to hypoxic conditions affected the redox potentials of all investigated organelles. Also, as reported previously, hypoxic conditions led to increased recombinant protein secretion. Finally, transcriptome and proteome datasets analysis provided novel insights into the short-term adaptation of the cells from normoxic to hypoxic conditions. In conclusion, improved production of therapeutic disulfide-bonded proteins in industrial settings can be achieved by tuning oxidative stress and redox homeostasis in the K. phaffii strains. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-30 |
AnnouncementXML | Submission_2025-01-30_02:37:10.188.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandra Maass |
SpeciesList | scientific name: Komagataella phaffii; NCBI TaxID: 460519; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-03 00:45:28 | ID requested | |
⏵ 1 | 2025-01-30 02:37:10 | announced | |
Publication List
Keyword List
submitter keyword: Komagataella phaffii, yeast, hypoxia, glutathione redox potential, chemostat cultivation |
Contact List
Dörte Becher |
contact affiliation | Department of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Greifswald, Germany |
contact email | dbecher@uni-greifswald.de |
lab head | |
Sandra Maass |
contact affiliation | University of Greifswald, Department for Microbial Proteomics |
contact email | sandra.maass@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055501
- Label: PRIDE project
- Name: Impact of oxygen availability on the organelle-specific redox potentials and stress in recombinant protein producing Komagataella phaffii