PXD055486

PXD055486 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the extracellular matrix in the porcine adrenal cortex
Description	The extracellular matrix (ECM) is a complex network of proteins that provides structural support and influences tissue boundaries, biomechanical properties, and cell polarity. This study analyzed the ECM profile of the porcine adrenal cortex's outer (OF = capsule + subcapsular + zona glomerulosa cells) and inner fractions (IF = zona fasciculata cells). Proteomic analysis of decellularized OF and IF samples identified 940 proteins, 22 collagens, 44 ECM glycoproteins, 9 proteoglycans, 20 ECM-regulators, 5 ECM associated proteins, 6 secreted factors, and 39 ECM candidates to compose the specific porcine adrenal matrisome. Among the ECM proteins identified, 113 are common to the OF and IF, while 16 were identified only in the OF and 21 in the IF. The analysis of protein abundance differences showed 3 proteins (Lamc1, Perlecan, Tgm2) significantly abundant in OF compared to IF. In IF, 11 proteins (Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Col6a6, Col14a1, Ecm1, Fga, Fgb, Fgg) were more abundant than OF. The comparative analysis of the quantification ECM proteins from the decellularized adrenal cortex of rats, humans, and pigs showed that in porcine samples, the ECM-quantified proteins are more abundant in the IF, while in rats and human samples, the more abundant ECM-quantified proteins are in the OF. These findings provide valuable insights into the potential of using pigs as a biomedical model and an essential tool for translational medical research.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:10:29.346.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055486
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jean Lucas Kremer
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-02 15:46:42	ID requested
⏵ 1	2025-07-13 16:10:30	announced

10.1021/acs.jproteome.5c00026;

10.6019/PXD055486;

Kremer JL, Ortega HS, Souza-Siqueira T, Blanes Angeli C, Kei Iwai L, Palmisano G, Lotfi CFP, Proteomic Analysis of the Extracellular Matrix in the Porcine Adrenal Cortex. J Proteome Res, 24(7):3300-3309(2025) [pubmed]

submitter keyword: adrenal cortex,Extracellular matrix, adrenal gland, porcine

Jean Lucas
contact affiliation	Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, São Paulo, Brazil, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP, 05508-000
contact email	jl.kremer@usp.br
lab head
Jean Lucas Kremer
contact affiliation	Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science, University of São Paulo
contact email	jl.kremer@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055486
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the extracellular matrix in the porcine adrenal cortex