PXD055478 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phages ZC01 and ZC03 require Type-IV Pilus for Pseudomonas aeruginosa infection and have a potential for therapeutic applications |
Description | There has been a growing interest in bacteriophages as therapeutic agents to treat multidrug-resistant bacterial infections, particularly those caused by the ESKAPE group. The present work aimed at expanding the microbiological and molecular characterization of phages ZC01 and ZC03 and investigating their efficacy in the control of P aeruginosa infection in an invertebrate animal model. These two phages were previously isolated from composting using P. aeruginosa strain PA14 as the enrichment host and had their genomes sequenced and annotated. ZC01 and ZC03 present, respectively, siphovirus and podovirus morphotypes. ZC01 was recently classified into the genus Abidjanvirus while ZC03 belongs to Zicotriavirus genus of the Schitoviridae N4-Like viruses. Through proteomics analysis, we identified virion structural proteins of ZC01 and ZC03, including a large virion-associated RNA polymerase that is characteristic of N4-Like viruses, some hypothetical proteins whose annotation should be changed to virion structural proteins and a putative peptidoglycan hydrolase. Phages ZC01 and ZC03 exhibit a limited yet distinct host range, with moderate to high Efficiency of Plating (EOP) values observed for a few P. aeruginosa clinical isolates. Phage susceptibility assays in PA14 mutant strains point to the type-IV pilus (T4P) as the primary receptor for phages ZC01 and ZC03 and that the major pilin (PilAPA14) is the T4P component recognized by these phages. Moreover, both phages significantly increase survival of Galleria mellonella larvae infected with PA14 strain. Taken together, these results underpin the therapeutic potential of these phages to treat infections by P. aeruginosa and lay the groundwork for a more detailed investigation of phage-bacteria specific recognition mechanisms. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_02:33:20.490.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claudia Blanes Angeli |
SpeciesList | scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-02 11:38:18 | ID requested | |
⏵ 1 | 2025-05-07 02:33:21 | announced | |
Publication List
Keyword List
submitter keyword: Pseudomonas aeruginosa |
PA14 |
phage therapy |
phage receptor |
Type-4 pilus |
Contact List
Aline Maria da Silva |
contact affiliation | Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil |
contact email | almsilva@iq.usp.br |
lab head | |
Claudia Blanes Angeli |
contact affiliation | University of Sao Paulo |
contact email | clauglin@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055478 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055478
- Label: PRIDE project
- Name: Phages ZC01 and ZC03 require Type-IV Pilus for Pseudomonas aeruginosa infection and have a potential for therapeutic applications