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PXD055478

PXD055478 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhages ZC01 and ZC03 require Type-IV Pilus for Pseudomonas aeruginosa infection and have a potential for therapeutic applications
DescriptionThere has been a growing interest in bacteriophages as therapeutic agents to treat multidrug-resistant bacterial infections, particularly those caused by the ESKAPE group. The present work aimed at expanding the microbiological and molecular characterization of phages ZC01 and ZC03 and investigating their efficacy in the control of P aeruginosa infection in an invertebrate animal model. These two phages were previously isolated from composting using P. aeruginosa strain PA14 as the enrichment host and had their genomes sequenced and annotated. ZC01 and ZC03 present, respectively, siphovirus and podovirus morphotypes. ZC01 was recently classified into the genus Abidjanvirus while ZC03 belongs to Zicotriavirus genus of the Schitoviridae N4-Like viruses. Through proteomics analysis, we identified virion structural proteins of ZC01 and ZC03, including a large virion-associated RNA polymerase that is characteristic of N4-Like viruses, some hypothetical proteins whose annotation should be changed to virion structural proteins and a putative peptidoglycan hydrolase. Phages ZC01 and ZC03 exhibit a limited yet distinct host range, with moderate to high Efficiency of Plating (EOP) values observed for a few P. aeruginosa clinical isolates. Phage susceptibility assays in PA14 mutant strains point to the type-IV pilus (T4P) as the primary receptor for phages ZC01 and ZC03 and that the major pilin (PilAPA14) is the T4P component recognized by these phages. Moreover, both phages significantly increase survival of Galleria mellonella larvae infected with PA14 strain. Taken together, these results underpin the therapeutic potential of these phages to treat infections by P. aeruginosa and lay the groundwork for a more detailed investigation of phage-bacteria specific recognition mechanisms.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_02:33:20.490.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudia Blanes Angeli
SpeciesList scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-02 11:38:18ID requested
12025-05-07 02:33:21announced
Publication List
10.1128/spectrum.01527-24;
Keyword List
submitter keyword: Pseudomonas aeruginosa
PA14
phage therapy
phage receptor
Type-4 pilus
Contact List
Aline Maria da Silva
contact affiliationDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil
contact emailalmsilva@iq.usp.br
lab head
Claudia Blanes Angeli
contact affiliationUniversity of Sao Paulo
contact emailclauglin@gmail.com
dataset submitter
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