PXD055477

PXD055477 is an original dataset announced via ProteomeXchange.

Title	Impact of Static Myoblast Loading on Protein Secretion Linked to tenocyte Migration
Description	Exercise has been shown to promote wound healing, including tendon repair. Myokines released from exercised muscles are believed to play a significant role in this process. In our previous study, we used an in vitro co-culture and loading model to demonstrate that 2% static loading of myoblasts increased the migration and proliferation of co-cultured tenocytes—two crucial aspects of wound healing. IGF-1, released from myoblasts in response to 2% static loading, was identified as a contributor to the increased proliferation. However, the factors responsible for enhanced migration were not clear. In the current study, we subjected myoblasts in single culture conditions to 2%, 5%, and 10% static loading and performed proteomic analysis on the cell supernatants. Gene Ontology (GO) analysis revealed that 2% static loading induced the secretion of NBL1, C5, and EFEMP1, which are associated with cell migration and motility. Further investigation by adding exogenous recombinant proteins to human tenocytes showed that NBL1 increased tenocyte migration, but not proliferation. This effect was not replicated by treatment with C5 and EFEMP1.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_07:35:09.805.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaochun Zhu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-02 10:27:22	ID requested
⏵ 1	2026-08-07 07:35:10	announced

10.1021/ACS.JPROTEOME.5C00068;

submitter keyword: tenocyte, myokines, wound healing,static loading, migration

Ludvig J. Backman
contact affiliation	Department of Medical and Translational Biology, Umeå University, Umeå SE-901 87, Sweden
contact email	ludvig.backman@umu.se
lab head
Shaochun Zhu
contact affiliation	Umea University
contact email	shaochunzhu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD055477

PRIDE project URI

• PRIDE
  1. PXD055477
     1. Label: PRIDE project
     2. Name: Impact of Static Myoblast Loading on Protein Secretion Linked to tenocyte Migration