PXD055461
PXD055461 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identifying direct interactome and substrates of human thioredoxins in specific cellular components with new cysteine-reactive enrichable chemical cross-linkers |
| Description | Identifying direct substrates of enzymes in specific cell compartment is challenging in methodology, but crucial for revealing unique cellular processes in different subcellular locations. Here we developed a comprehensive live cell cross-linking coupled mass spectrometry strategy to identify direct interactome of redox enzymes in subcellular level. We chemically synthesized two new enrichable bis-vinyl sulfone chemical cross-linker ePDES1 and ePDES2 with alkyne group, and firstly established two-step IMAC enrichment method for high efficiency cross-linking peptide enrichment. Based on high quality ePDES1 and ePDES2 cross-linking spectra, our method achieved identifying hundreds of direct interacting proteins and substrates cross-linking to active cysteine sites of TXN1 both in cytoplasm and nuclei and TXN2 in mitochondria. It is interesting to simultaneously identify proteins cross-linked to TXN1 active Cys32 and Cys73 responsible for denitrosylation and transnitrosylation, respectively. Our method firstly identified direct interactome and substrates of oxidoreductases in subcellular level, providing resources for precisely studying redox homeostasis in specific cell compartment. |
| HostingRepository | iProX |
| AnnounceDate | 2024-09-02 |
| AnnouncementXML | Submission_2025-06-11_22:51:02.901.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ting Wu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-09-01 23:11:43 | ID requested | |
| ⏵ 1 | 2025-06-11 22:51:03 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: XL-MS, TXN1, TXN2, Two-step IMAC enrichment, Nucleous, Mitochondria |
Contact List
| Long Zhang | |
|---|---|
| contact affiliation | Life Science Institute of Zhejiang University |
| contact email | l_zhang@zju.eu.cn |
| lab head | |
| Ting Wu | |
| contact affiliation | Life Science Institute of Zhejiang University |
| contact email | 928797084@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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