PXD055450

PXD055450 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation regulates the chromatin remodeler SMARCAD1 in nucleosome binding, ATP hydrolysis, and histone exchange
Description	Maintaining the dynamic structure of chromatin is critical for regulating the cellular processes that require access to the DNA template, such as DNA damage repair, transcription, and replication. Histone chaperones and ATP-dependent chromatin remodeling factors facilitate transitions in chromatin structure by assembling and positioning nucleosomes through a variety of enzymatic activities. SMARCAD1 is a unique chromatin remodeler that combines the ATP-dependent ability to exchange histones, with the chaperone-like activity of nucleosome deposition. We have shown previously that phosphorylated SMARCAD1 exhibits reduced binding to nucleosomes. However, it is unknown how phosphorylation affects SMARCAD1’s ability to perform its various enzymatic activities. Here we use mutational analysis, activity assays, and mass spectrometry, to probe SMARCAD1 regulation and to investigate the role of its flexible N-terminal region. We show that phosphorylation affects SMARCAD1 binding to nucleosomes, DNA, and histone H2A-H2B, as well as ATP hydrolysis and histone exchange. Conversely, we report that phosphorylation is inconsequential for histone H3-H4 binding and nucleosome assembly. In addition, the SMARCAD1 N-terminal region is revealed to be critical for nucleosome assembly and histone exchange. Together, this work examines the intricacies of how phosphorylation governs SMARCAD1 activity and provides insight into its complex regulation and diverse activities.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:34:13.491.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055450
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Briana Aboulache
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-01 21:55:40	ID requested
⏵ 1	2025-05-07 02:34:14	announced

10.6019/PXD055450;

Aboulache BL, Hoitsma NM, Luger K, Phosphorylation regulates the chromatin remodeler SMARCAD1 in nucleosome binding, ATP hydrolysis, and histone exchange. J Biol Chem, 300(12):107893(2024) [pubmed]

10.1016/j.jbc.2024.107893;

submitter keyword: nucleosome, nucleosome assembly, chromatin remodeling, histone exchange, ATP hydrolysis, histone chaperone,SMARCAD1, phosphorylation, post-translational modification (PTM)

Karolin Luger
contact affiliation	University of Colorado Boulder
contact email	Karolin.Luger@colorado.edu
lab head
Briana Aboulache
contact affiliation	Graduate Student
contact email	briana.aboulache@colorado.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055450
     1. Label: PRIDE project
     2. Name: Phosphorylation regulates the chromatin remodeler SMARCAD1 in nucleosome binding, ATP hydrolysis, and histone exchange