PXD055429
PXD055429 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunoprecipitation (IP) combined with LC-MS/MS to identify potential plant targets of BcCELP1 |
Description | The raw MS data were analyzed and searched against the target protein database using Maxquant (1.6.2.10). Only peptides identified with high confidence (with enzyme specificity set to trypsin; maximum missed cleavages set to 2; precursor ion mass tolerance set to 20 ppm, and MS/MS tolerance 20 ppm) were chosen for downstream protein identification analysis. To confirm the interaction between BcCELP1 and NbRACK1, a Co-IP assay was conducted as follows: pCNG-BcCELP1, pCNG-EV and pCNF3-NbRACK1 constructs were transiently co-expressed by agroinfiltration of 4-5 week-old N. benthamiana leaves. Total proteins were extracted from leaves using a cell lysis buffer, and subjected to anti-GFP agarose beads IP (chromotek) as previously described45. Eluted proteins were subjected to immunoblot analysis using anti-GFP antibodies and anti-FALG antibodies. Total proteins were loaded as an input control. |
HostingRepository | iProX |
AnnounceDate | 2024-08-28 |
AnnouncementXML | Submission_2024-09-01_19:29:08.842.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kai Bi |
SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 240 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-09-01 19:28:58 | ID requested | |
⏵ 1 | 2024-09-01 19:29:09 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: BcCELP1, Botrytis, plant interactors |
Contact List
Kai Bi | |
---|---|
contact affiliation | Wuhan Polytechnic University |
contact email | bikai123@sina.com |
lab head | |
Kai Bi | |
contact affiliation | Wuhan Polytechnic University |
contact email | bikai123@sina.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |