⮝ Full datasets listing

PXD055429

PXD055429 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunoprecipitation (IP) combined with LC-MS/MS to identify potential plant targets of BcCELP1
DescriptionThe raw MS data were analyzed and searched against the target protein database using Maxquant (1.6.2.10). Only peptides identified with high confidence (with enzyme specificity set to trypsin; maximum missed cleavages set to 2; precursor ion mass tolerance set to 20 ppm, and MS/MS tolerance 20 ppm) were chosen for downstream protein identification analysis. To confirm the interaction between BcCELP1 and NbRACK1, a Co-IP assay was conducted as follows: pCNG-BcCELP1, pCNG-EV and pCNF3-NbRACK1 constructs were transiently co-expressed by agroinfiltration of 4-5 week-old N. benthamiana leaves. Total proteins were extracted from leaves using a cell lysis buffer, and subjected to anti-GFP agarose beads IP (chromotek) as previously described45. Eluted proteins were subjected to immunoblot analysis using anti-GFP antibodies and anti-FALG antibodies. Total proteins were loaded as an input control.
HostingRepositoryiProX
AnnounceDate2024-08-28
AnnouncementXMLSubmission_2024-09-01_19:29:08.842.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKai Bi
SpeciesList scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 240
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-01 19:28:58ID requested
12024-09-01 19:29:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: BcCELP1, Botrytis, plant interactors
Contact List
Kai Bi
contact affiliationWuhan Polytechnic University
contact emailbikai123@sina.com
lab head
Kai Bi
contact affiliationWuhan Polytechnic University
contact emailbikai123@sina.com
dataset submitter
Full Dataset Link List
iProX dataset URI