PXD055417

PXD055417 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome of plasma exosome reveals three proteins with potential diagnostic value for Mycobacterium bovis infection in cows
Description	Bovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a zoonotic disease that poses a significant threat to both cattle and human health. Currently, the immunological diagnosis using tuberculin tuberculin skin testing (TST) is widely employed for bTB surveillance. There is an urgent need for on-farm inspection indicators or strategies for bTB that utilize easily accessible fluid samples. Previously, we presented a comprehensive proteomic overview of plasma-derived exosomes from M. bovis-infected cows. In this study, we used other two parallel cohorts from two farms to illustrate the expression characteristics of infected cows and explore potential bTB indicators using plasma exosome proteome profiling. Cohort A included 15 negative and 22 positive samples, while cohort B included 28 negative and 40 positive samples. Among the 777 identified proteins, 217 in cohort A and 233 in cohort B showed differential expression between negative and positive samples. Of these, 47 proteins exhibited consistent dysregulation in both cohorts and were significantly enriched in tuberculosis, antigen processing and presentation pathways. Additionally, three proteins, HSPA8, B2M and HRG, were identified and verified as bTB indicators through Lasso regression analysis, western blot and ELISA validation. This study enhances us understanding of the dynamic expression change in plasma exosomes following M. bovis infection and provides technical insights for bTB detection based on clinical fluid samples.
HostingRepository	iProX
AnnounceDate	2024-08-28
AnnouncementXML	Submission_2024-09-01_18:50:10.738.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Zhang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-01 18:49:52	ID requested
⏵ 1	2024-09-01 18:50:11	announced

Dataset with its publication pending

submitter keyword: bovine tuberculosis, plasma exosome, proteome, diagnostic indicators

Ping Xu
contact affiliation	Beijing Proteome Research Center
contact email	xuping_bprc@126.com
lab head
Yao Zhang
contact affiliation	Beijing Proteome Research Center
contact email	zhangyaowsw@163.com
dataset submitter

iProX dataset URI