PXD055387

PXD055387 is an original dataset announced via ProteomeXchange.

Title	Establishing an activity-based protein profiling platform to screen in situ kinase inhibitor engagement
Description	Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44 is a chemical probe based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cells with a drug (candidate) results in competition of XO44 binding, which can be interpreted as in situ target engagement. Here, a workflow is presented which is optimized for kinome coverage, reproducibility and data processing. This dataset pertains to the selectivity testing of Midostaurin in MV4-11 cells. (Phosphoproteomics)
HostingRepository	PRIDE
AnnounceDate	2025-04-09
AnnouncementXML	Submission_2025-04-09_01:23:52.191.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Berend Gagestein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-30 06:53:31	ID requested
⏵ 1	2025-04-09 01:23:52	announced

10.1016/j.mcpro.2025.100961;

R, ü, egger J, Gagestein B, Janssen APA, Valeanu A, Mori AL, van der Peet M, Boutkan MS, Florea BI, Henneman AA, Hochstrasser R, Wang H, Westwood P, Topp A, Gomez Barila PM, Medema JP, Jimenez CR, Woersdoerfer B, Kirchner S, Zhang JD, Grether U, Rufer AC, van der Stelt M, CellEKT: A Robust Chemical Proteomics Workflow to Profile Cellular Target Engagement of Kinase Inhibitors. Mol Cell Proteomics, 24(6):100961(2025) [pubmed]

submitter keyword: drug discovery, kinase, phosphoproteomics,ABPP

Mario van der Stelt
contact affiliation	Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Berend Gagestein
contact affiliation	Leiden University
contact email	berendgagestein@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD055387

PRIDE project URI

• PRIDE
  1. PXD055387
     1. Label: PRIDE project
     2. Name: Establishing an activity-based protein profiling platform to screen in situ kinase inhibitor engagement