PXD055384

PXD055384 is an original dataset announced via ProteomeXchange.

Title	MEATiCode: A COMPREHENSIVE PROTEOMIC LC-MS/MS METHOD FOR SIMULTANEOUS SPECIES IDENTIFICATION IN MEAT AUTHENTICATION
Description	The issue of food fraud within the meat industry represents a significant challenge with the potential to impact consumer trust, market stability, and public health. The application of traditional methods, such as DNA barcoding, is constrained, particularly in the context of processed foods, where DNA degradation is a common occurrence. This paper introduces the workflow MEATiCode, a comprehensive proteomic LC-MS/MS method for the simultaneous identification of species in meat authentication. A simple sample preparation procedure, comprising extraction, digestion and desalting, was employed, followed by the application of liquid chromatography tandem mass spectrometry (LC-MS/MS). This enabled the differentiation of peptides and proteins between beef, pork, chicken and lamb species with a limit of detection of 0.5%. The method demonstrated high sensitivity and specificity, effectively identifying species-specific peptides and proteins. The study provides a comprehensive account of the development of the MEATiCode database, which incorporates species-specific peptides and integrates this database with Mascot Engine and Proteome Discoverer for the purpose of detecting potential fraud in processed meat products. The application of the MEATiCode method to a range of meat products demonstrated its efficacy, achieving high sensitivity and reliability in the detection of adulteration, even in highly processed or cooked meats.
HostingRepository	PRIDE
AnnounceDate	2025-05-01
AnnouncementXML	Submission_2025-05-01_05:47:25.606.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055384
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Renata Duft
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Cervus elaphus hippelaphus; NCBI TaxID: 46360; scientific name: Ovis aries; NCBI TaxID: 9940; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Meleagris gallopavo silvestris; NCBI TaxID: 109974;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-30 06:18:07	ID requested
⏵ 1	2025-05-01 05:47:26	announced

10.1016/J.FOODCHEM.2025.144231;

10.6019/PXD055384;

submitter keyword: meat authentication,proteomics, liquid chromatography mass spectrometry, food fraud

Julian L. Griffin
contact affiliation	University of Aberdeen
contact email	jules.griffin@abdn.ac.uk
lab head
Renata Duft
contact affiliation	University of Aberdeen
contact email	renata.garbelliniduft@abdn.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055384
     1. Label: PRIDE project
     2. Name: MEATiCode: A COMPREHENSIVE PROTEOMIC LC-MS/MS METHOD FOR SIMULTANEOUS SPECIES IDENTIFICATION IN MEAT AUTHENTICATION