PXD055360

PXD055360 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals blunted effect of sprint interval training by vitamin C and E treatment in both type 1 and type 2 muscle fibres
Description	We hypothesized that SIT causes oxidative stress preferentially in fast-twitch type 2-fibres and that this stress constitutes an important trigger of mitochondrial adaptations towards increased oxidative capacity and hence improved muscular endurance. In addition, we hypothesized that the SIT-induced signalling involves an initial ROS-dependent increase in SR Ca2+ leak followed by adaptive changes in the expression of SR Ca2+-handling proteins. To test these hypotheses, two groups of young males performed three weeks of SIT, one group received antioxidants in the form of high dosage of vitamin C and E and the other placebo. In addition to physiological exercise performance tests, we focus on changes in ROS-, SR Ca2+-, and mitochondria-related gene expression after the initial SIT session and fibre type-dependent protein expression after the last SIT session.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_04:08:21.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Murgia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-29 08:25:12	ID requested
⏵ 1	2025-07-29 04:08:22	announced

10.1113/jp288638;

Wyckelsma VL, Murgia M, Kamandulis S, Gastaldello S, Brazaitis M, Snieckus A, Eimantas N, P, ä, ä, suke M, Edman S, Apro W, Andersson DC, Westerblad H, Venckunas T, weeks of sprint interval training preferentially in human type 2 muscle fibres. J Physiol, ():(2025) [pubmed]

submitter keyword: proteomics, sprint interval training,Muscle fibers

Håkan Westerblad
contact affiliation	Karolinska Institute, Department of Physiology and Pharmacology
contact email	hakan.westerblad@ki.se
lab head
Marta Murgia
contact affiliation	MPI Biochemistry
contact email	mmurgia@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD055360

PRIDE project URI

• PRIDE
  1. PXD055360
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals blunted effect of sprint interval training by vitamin C and E treatment in both type 1 and type 2 muscle fibres