PXD055324

PXD055324 is an original dataset announced via ProteomeXchange.

Title	Protein mass spectrum analysis of serum from CKD and NC mice
Description	As for serum (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. We utilized immunoaffinity depletion of serum high-abundance proteins. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionation with reversed-phase (RP) chromatography and mass spectrometry analysis. Mass Spectrometry analysis included Easy nLC, LC-MS/MS and data analysis. Differentially expressed proteins between two groups were determined using the t test (the threshold values were set as P < 0.05 and 1.5-fold change).
HostingRepository	iProX
AnnounceDate	2024-08-25
AnnouncementXML	Submission_2025-03-06_23:27:41.369.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meiqiu Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 20:51:16	ID requested
⏵ 1	2025-03-06 23:27:42	announced

Wang M, You L, He X, Peng Y, Wang R, Zhang Z, Shu J, Zhang P, Sun X, Jia L, Xia Z, Ji C, Gao C, Multiomics Analysis Reveals Therapeutic Targets for Chronic Kidney Disease With Sarcopenia. J Cachexia Sarcopenia Muscle, 16(1):e13696(2025) [pubmed]

submitter keyword: chronic kidney disease, serum, mice

Chunlin Gao
contact affiliation	Nanjing University
contact email	shuangmu34@163.com
lab head
Meiqiu Wang
contact affiliation	Nanjing University
contact email	1481700382@qq.com
dataset submitter

iProX dataset URI