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PXD055324

PXD055324 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein mass spectrum analysis of serum from CKD and NC mice
DescriptionAs for serum (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. We utilized immunoaffinity depletion of serum high-abundance proteins. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionation with reversed-phase (RP) chromatography and mass spectrometry analysis. Mass Spectrometry analysis included Easy nLC, LC-MS/MS and data analysis. Differentially expressed proteins between two groups were determined using the t test (the threshold values were set as P < 0.05 and 1.5-fold change).
HostingRepositoryiProX
AnnounceDate2024-08-25
AnnouncementXMLSubmission_2025-03-06_23:27:41.369.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeiqiu Wang
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-28 20:51:16ID requested
12025-03-06 23:27:42announced
Publication List
Wang M, You L, He X, Peng Y, Wang R, Zhang Z, Shu J, Zhang P, Sun X, Jia L, Xia Z, Ji C, Gao C, Multiomics Analysis Reveals Therapeutic Targets for Chronic Kidney Disease With Sarcopenia. J Cachexia Sarcopenia Muscle, 16(1):e13696(2025) [pubmed]
Keyword List
submitter keyword: chronic kidney disease, serum, mice
Contact List
Chunlin Gao
contact affiliationNanjing University
contact emailshuangmu34@163.com
lab head
Meiqiu Wang
contact affiliationNanjing University
contact email1481700382@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI