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PXD055302

PXD055302 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBMP9 Secretome of hHiPC LC-MS/MS
DescriptionTo investigate BMP9/ALK1 signaling in human highly proliferative cells (hHiPC), we selected one primary population of hHiPC from 3 individuals and studied the secretome responses to BMP9 treatment. Proteins were collected in hHiPC treated conditioned media (CM) for mass spectrometry analysis. Proteomic analysis of the hHiPC secretome following BMP9 treatment demonstrates that the secreted proteins, sclerostin (SOST), meflin/immunoglobulin superfamily containing leucine rich repeat (ISLR), and insulin-like growth factor binding protein-3 (IGFBP3), are novel downstream targets of BMP9/ALK1-mediated signaling. Consistent with this hypothesis, lentiviral shRNA and pharmacological inhibition of ALK1 in hHiPCs suppressed secretion of SOST, ISLR, and IGFBP3 following BMP9 treatment as measured by secretome LC-MS/MS.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_02:21:35.302.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichayla Moore
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-28 14:30:35ID requested
12025-05-07 02:21:36announced
Publication List
Moore M, Ryzhov S, Sawyer DB, Gartner C, Vary CPH, ALK1 Signaling in Human Cardiac Progenitor Cells Promotes a Pro-angiogenic Secretome. J Cell Signal, 5(3):122-142(2024) [pubmed]
10.33696/signaling.5.119;
Keyword List
submitter keyword: Human, Heart, Secretome
Contact List
Calvin Vary
contact affiliationProteomics Core, MaineHealth Institute for Research
contact emailcalvin.vary@mainehealth.org
lab head
Michayla Moore
contact affiliationUniversity of Maine, MaineHealth Institute for Research
contact emailmichayla.moore@maine.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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