PXD055302 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BMP9 Secretome of hHiPC LC-MS/MS |
Description | To investigate BMP9/ALK1 signaling in human highly proliferative cells (hHiPC), we selected one primary population of hHiPC from 3 individuals and studied the secretome responses to BMP9 treatment. Proteins were collected in hHiPC treated conditioned media (CM) for mass spectrometry analysis. Proteomic analysis of the hHiPC secretome following BMP9 treatment demonstrates that the secreted proteins, sclerostin (SOST), meflin/immunoglobulin superfamily containing leucine rich repeat (ISLR), and insulin-like growth factor binding protein-3 (IGFBP3), are novel downstream targets of BMP9/ALK1-mediated signaling. Consistent with this hypothesis, lentiviral shRNA and pharmacological inhibition of ALK1 in hHiPCs suppressed secretion of SOST, ISLR, and IGFBP3 following BMP9 treatment as measured by secretome LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_02:21:35.302.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michayla Moore |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-28 14:30:35 | ID requested | |
⏵ 1 | 2025-05-07 02:21:36 | announced | |
Publication List
Moore M, Ryzhov S, Sawyer DB, Gartner C, Vary CPH, ALK1 Signaling in Human Cardiac Progenitor Cells Promotes a Pro-angiogenic Secretome. J Cell Signal, 5(3):122-142(2024) [pubmed] |
10.33696/signaling.5.119; |
Keyword List
submitter keyword: Human, Heart, Secretome |
Contact List
Calvin Vary |
contact affiliation | Proteomics Core, MaineHealth Institute for Research |
contact email | calvin.vary@mainehealth.org |
lab head | |
Michayla Moore |
contact affiliation | University of Maine, MaineHealth Institute for Research |
contact email | michayla.moore@maine.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055302 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055302
- Label: PRIDE project
- Name: BMP9 Secretome of hHiPC LC-MS/MS