PXD055302

PXD055302 is an original dataset announced via ProteomeXchange.

Title	BMP9 Secretome of hHiPC LC-MS/MS
Description	To investigate BMP9/ALK1 signaling in human highly proliferative cells (hHiPC), we selected one primary population of hHiPC from 3 individuals and studied the secretome responses to BMP9 treatment. Proteins were collected in hHiPC treated conditioned media (CM) for mass spectrometry analysis. Proteomic analysis of the hHiPC secretome following BMP9 treatment demonstrates that the secreted proteins, sclerostin (SOST), meflin/immunoglobulin superfamily containing leucine rich repeat (ISLR), and insulin-like growth factor binding protein-3 (IGFBP3), are novel downstream targets of BMP9/ALK1-mediated signaling. Consistent with this hypothesis, lentiviral shRNA and pharmacological inhibition of ALK1 in hHiPCs suppressed secretion of SOST, ISLR, and IGFBP3 following BMP9 treatment as measured by secretome LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:21:35.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michayla Moore
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 14:30:35	ID requested
⏵ 1	2025-05-07 02:21:36	announced

Moore M, Ryzhov S, Sawyer DB, Gartner C, Vary CPH, ALK1 Signaling in Human Cardiac Progenitor Cells Promotes a Pro-angiogenic Secretome. J Cell Signal, 5(3):122-142(2024) [pubmed]

10.33696/signaling.5.119;

submitter keyword: Human, Heart, Secretome

Calvin Vary
contact affiliation	Proteomics Core, MaineHealth Institute for Research
contact email	calvin.vary@mainehealth.org
lab head
Michayla Moore
contact affiliation	University of Maine, MaineHealth Institute for Research
contact email	michayla.moore@maine.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055302

PRIDE project URI

• PRIDE
  1. PXD055302
     1. Label: PRIDE project
     2. Name: BMP9 Secretome of hHiPC LC-MS/MS