<<< Full experiment listing

PXD055293

PXD055293 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSubfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana
DescriptionThe calcium-dependent protein kinase CPK28 is a regulator of immune homeostasis in multiple plant species. Here, we used a proteomics approach to uncover CPK28-associated proteins. We found that CPK28 associates with subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39, and trans-phosphorylates RAF26 and RAF39. Metazoan Raf kinases function in mitogen-activated protein kinase (MAPK) cascades as MAPK kinase kinases (MKKKs). Although Raf-like kinases share some features with MKKKs, we found that MRK1, RAF26, and RAF39 are unable to trans-phosphorylate any of the 10 Arabidopsis MKKs. We show that MRK1, RAF26, and RAF39 localize to the cytosol and endomembranes, and we define redundant roles for these kinases in stomatal opening, immune-triggered reactive oxygen species (ROS) production, and resistance to a bacterial pathogen. Overall, our study suggests that C7 Raf-like kinases associate with and are phosphorylated by CPK28, function redundantly in stomatal opening and immunity, and possess substrate specificities distinct from canonical MKKKs.
HostingRepositoryPRIDE
AnnounceDate2024-10-01
AnnouncementXMLSubmission_2024-10-01_07:15:21.134.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD055293
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJan Sklenar
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-28 06:14:53ID requested
12024-10-01 07:15:21announced
Publication List
10.1101/2023.11.29.569073;
10.6019/PXD055293;
Keyword List
submitter keyword: CPK28,Arabidopsis, MRK1
Contact List
Frank Menke
contact affiliationProteomics Support Group, The Sainsbury Laboratory, Norwich, UK
contact emailfrank.menke@tsl.ac.uk
lab head
Jan Sklenar
contact affiliationThe Sainsbury Laboratory, Norwich, UK
contact emailjan.sklenar@tsl.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD055293
PRIDE project URI
Repository Record List
[ + ]