PXD055293

PXD055293 is an original dataset announced via ProteomeXchange.

Title	Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana
Description	The calcium-dependent protein kinase CPK28 is a regulator of immune homeostasis in multiple plant species. Here, we used a proteomics approach to uncover CPK28-associated proteins. We found that CPK28 associates with subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39, and trans-phosphorylates RAF26 and RAF39. Metazoan Raf kinases function in mitogen-activated protein kinase (MAPK) cascades as MAPK kinase kinases (MKKKs). Although Raf-like kinases share some features with MKKKs, we found that MRK1, RAF26, and RAF39 are unable to trans-phosphorylate any of the 10 Arabidopsis MKKs. We show that MRK1, RAF26, and RAF39 localize to the cytosol and endomembranes, and we define redundant roles for these kinases in stomatal opening, immune-triggered reactive oxygen species (ROS) production, and resistance to a bacterial pathogen. Overall, our study suggests that C7 Raf-like kinases associate with and are phosphorylated by CPK28, function redundantly in stomatal opening and immunity, and possess substrate specificities distinct from canonical MKKKs.
HostingRepository	PRIDE
AnnounceDate	2024-10-01
AnnouncementXML	Submission_2024-10-01_07:15:21.134.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055293
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Sklenar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 06:14:53	ID requested
⏵ 1	2024-10-01 07:15:21	announced

10.1101/2023.11.29.569073;

10.6019/PXD055293;

submitter keyword: CPK28,Arabidopsis, MRK1

Frank Menke
contact affiliation	Proteomics Support Group, The Sainsbury Laboratory, Norwich, UK
contact email	frank.menke@tsl.ac.uk
lab head
Jan Sklenar
contact affiliation	The Sainsbury Laboratory, Norwich, UK
contact email	jan.sklenar@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055293
     1. Label: PRIDE project
     2. Name: Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana