PXD055281

PXD055281 is an original dataset announced via ProteomeXchange.

Title	Revisiting the relevance of the accumulation of apolipoprotein E (APOE) in mitochondria-associated ER membranes: Investi-gation of hepatic protein-protein interactions of APOE with LONP1, TOMM40 and VDAC1
Description	Investigating the subcellular distribution and protein interaction of APOE and the modulation by cellular stress signals, we aimed to contribute to the elucidation of putative novel functions. We identified APOE as hepatic mitochondria-associated ER-membranes (MAM) protein, where it most likely interacts with the mitochondrial proteins LONP1, TOMM40 and VDAC1. We pro-pose that the interaction with TOMM40 and VDAC1 enables specific mitochondria-ER contacts with so far unknown functional relevance. However, MAM residing APOE may help in unfolded protein response possibly by co-operation with LONP1 at the interface of mitochondria and ER membranes.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_02:05:35.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 03:23:16	ID requested
⏵ 1	2025-05-07 02:05:36	announced

10.3390/ijms251910597;

Rueter J, Rimbach G, Bilke S, Tholey A, Huebbe P, Readdressing the Localization of Apolipoprotein E (APOE) in Mitochondria-Associated Endoplasmic Reticulum (ER) Membranes (MAMs): An Investigation of the Hepatic Protein-Protein Interactions of APOE with the Mitochondrial Proteins Lon Protease (LONP1), Mitochondrial Import Receptor Subunit TOM40 (TOMM40) and Voltage-Dependent Anion-Selective Channel 1 (VDAC1). Int J Mol Sci, 25(19):(2024) [pubmed]

submitter keyword: mitochondria-ER contacts (MERC), MAM, stress response, proteome analysis, subcellular distribution, liver, APOE4

Andreas Tholey
contact affiliation	Institut für experimentelle Medizin Systematische Proteomics & Bioanalytik Niemannsweg 11 24105 Kiel
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055281

PRIDE project URI

• PRIDE
  1. PXD055281
     1. Label: PRIDE project
     2. Name: Revisiting the relevance of the accumulation of apolipoprotein E (APOE) in mitochondria-associated ER membranes: Investi-gation of hepatic protein-protein interactions of APOE with LONP1, TOMM40 and VDAC1