PXD055276

PXD055276 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mouse bone marrow fibrosis LC-MS/MS
Description	Bone marrow fibrosis (BMF) disrupts normal blood cell production, and excessive activation of the TGF-β signaling pathway is considered a key factor in the progression of the disease. Therefore, regulating TGF-β secretion is crucial for reversing fibrotic conditions. Histone deacetylase inhibitors (HDACis) are one such potential regulator, as they can influence TGF-β expression. In a previous study, we developed a selective HDAC6 inhibitor, J22352, targeting pulmonary fibrosis, but its effects on BMF were not previously examined. This study aimed to evaluate J22352's impact on bone marrow-derived myofibroblasts. We found that J22352 significantly reduced cell viability, triggered apoptosis, and suppressed extracellular matrix (ECM) accumulation. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified 334 differentially expressed proteins (DEPs) linked to apoptosis, programmed cell death, ECM deposition, and collagen formation. These findings indicate that J22352 effectively mitigates BMF by inducing apoptosis and reducing ECM buildup. This study highlights J22352 as a promising selective HDAC6 inhibitor that could potentially slow BMF progression, offering new possibilities for clinical applications.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:47:05.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiao-Hsu Ke
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-28 01:33:35	ID requested
⏵ 1	2025-08-24 16:47:06	announced

Publication List

Ke CH, Huang ME, Wu HY, Yu CW, Lin SL, Lin SP, Yu SH, Huang CH, Lin CS, High-selective HDAC6 inhibitor alleviates bone marrow fibrosis through inhibiting collagen formation and extracellular matrix deposition. Sci Rep, 15(1):28105(2025) [pubmed]
10.1038/s41598-025-08384-6;

Ke CH, Huang ME, Wu HY, Yu CW, Lin SL, Lin SP, Yu SH, Huang CH, Lin CS, High-selective HDAC6 inhibitor alleviates bone marrow fibrosis through inhibiting collagen formation and extracellular matrix deposition. Sci Rep, 15(1):28105(2025) [pubmed]

10.1038/s41598-025-08384-6;

Keyword List

submitter keyword: Mouse, bone marrow fibrosis, LC-MS/MS

submitter keyword: Mouse, bone marrow fibrosis, LC-MS/MS

Contact List

Chen-Si Lin
contact affiliation	Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University
contact email	cslin100@ntu.edu.tw
lab head
Chiao-Hsu Ke
contact affiliation	National Taiwan University
contact email	f08629002@ntu.edu.tw
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD055276
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD055276

PRIDE project URI

Repository Record List

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