PXD055265

PXD055265 is an original dataset announced via ProteomeXchange.

Title	DNMT3A regulates megakaryocyte-biased HSC fate decisions
Description	We characterized the role of the DNA methyltransferase DNMT3A in the fate decisions of murine megakaryocyte-biased HSCs and in the differentiation of megakaryocytes and platelets. In this experiment, we isolated washed platelets from 4 WT and 4 Dnmt3a+/– germline mice (2 males and 2 females per group) and subjected them to proteomic analysis by mass-spectrometry. We sought to identify changes at the protein level that occur in platelets due to aberrant DNA methylation during the differentiation of upstream hematopoietic cells.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_16:10:43.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antrix Jain
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-27 13:09:12	ID requested
⏵ 1	2025-06-08 16:10:43	announced

10.1182/bloodadvances.2024015061;

Waldvogel SM, Camacho V, Fan D, Guzman AG, Garcia-Martell A, Khabusheva E, Pridgen JR, De La Fuente J, Rau R, Laidman AG, Barrachina MN, Carminita E, Courson JA, Williamson MR, Hsu JI, Chen CW, Reyes J, Pradhan S, Rumbaut RE, Burns AR, Deneen B, Su J, Machlus KR, Goodell MA, DNMT3A regulates murine megakaryocyte-biased hematopoietic stem cell fate decisions. Blood Adv, 9(9):2285-2299(2025) [pubmed]

submitter keyword: platelets,Megakaryocytes, DNA methylation, DNMT3A

Margaret A. Goodel
contact affiliation	Molecular and Cellular Biology Baylor College of Medicine Houston, Texas
contact email	goodell@bcm.edu
lab head
Antrix Jain
contact affiliation	Baylor College of Medicine
contact email	antrixj@bcm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD055265

PRIDE project URI

• PRIDE
  1. PXD055265
     1. Label: PRIDE project
     2. Name: DNMT3A regulates megakaryocyte-biased HSC fate decisions