PXD055263

PXD055263 is an original dataset announced via ProteomeXchange.

Title	A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity
Description	Outer mitochondrial membrane (OMM) proteins communicate with the cytosol and other organelles including the endoplasmic reticulum (ER). This communication is important in thermogenic adipocytes to increase energy expenditure that controls body temperature and weight. However, the regulatory mechanisms of OMM protein insertion are poorly understood. Herein, stress-induced cytosolic chaperone PPID (peptidyl-prolyl isomerase D/cyclophilin 40/Cyp40) drives OMM insertion of the mitochondrial import receptor TOM70 regulating body temperature and weight in obese mice, and respiratory/thermogenic function in brown adipocytes. PPID PPIase activity and C-terminal tetratricopeptide repeats (TPR), which show specificity towards TOM70 core and C-tail domains, facilitate OMM insertion. Our results provide an unprecedented role for ER-stress-activated chaperones in controlling energy metabolism through a selective OMM protein insertion mechanism with implications in adaptation to cold temperatures and high-calorie diets.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-02_16:01:44.751.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055263
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pere Puigserver
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-27 12:44:18	ID requested
⏵ 1	2024-10-02 16:01:45	announced

10.6019/PXD055263;

submitter keyword: Mitochondria, TOM70,Chaparone, Obesity

Pere Puigserver
contact affiliation	Dana-Farber Cancer Institute, Dept. Cancer Biology; Harvard Medical School, Dept. Cell Biology
contact email	Pere_Puigserver@dfci.harvard.edu
lab head
Pere Puigserver
contact affiliation	Dana-Farber Cancer Intitute, Harvard Medical School
contact email	pere_puigserver@dfci.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055263
     1. Label: PRIDE project
     2. Name: A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity