PXD055254

PXD055254 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of a novel iPSC-derived 3D brain tissue model (3BTM) of Alzheimer’s disease
Description	Stem-cell-based in vitro models offer promising potential to elucidate pathomechanisms underlying neurological diseases such as Alzheimer’s disease. However, harnessing this potential is currently impaired by low reproducibility, maturity, and cell-type diversity of models and lack of AD-relevant pathologies. Therefore, we developed a novel 3D cortical tissue model containing neurons, astrocytes, and microglia with high reproducibility, maturity, and viability. Upon knock-in of AD-causing APP mutations we found typical pathologies such as time- and cell-type-dependent accumulation and aggregation of Aβ, increased phosphor-tau levels, and microglia activation. To get an unbiased overview of pathology-related changes, we performed proteome analysis of our cultures containing different cell types at different timepoints.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-23_14:20:00.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-27 07:57:04	ID requested
⏵ 1	2026-02-23 14:20:01	announced

Dataset with its publication pending

submitter keyword: DIA, iPSC, label-free,human, cortical tissue model

Stefan Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen-Str. 17, 81377 München, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD055254

PRIDE project URI

• PRIDE
  1. PXD055254
     1. Label: PRIDE project
     2. Name: Proteomic analysis of a novel iPSC-derived 3D brain tissue model (3BTM) of Alzheimer’s disease