PXD055248 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparison of Cerium nanoparticles toxicity against Bacillus subtilis grown in two different types of biofilm |
| Description | The project is focused on the soil bacterium model Bacillus subtilis that is one of the essential components of the rhizosphere. In its natural ecosystem, this bacterium develops in the form of a biofilm, particularly around plant roots. Two types of biofilm that are closer to its natural environment than the usual liquid type culture, have been assayed, pellicle biofilm and swarming. We then used the power of shotgun proteomics and bioinformatics tools to analyze in details these two somehow similar modes of cultures. Several physiological and metabolic patterns including proteins implicated in sporulation have shown significant differences triggered by the growth culture mode. The effect of two types of nanoparticles SiO2 and the widely used nanoceria (cerium oxide nanoparticles) has then been studied in the same way. This allowed us to investigate in details the response of the bacteria to the cerium oxide nanoparticles and especially to focus on the differences observed depending on the biofilm type, including proteins associated with iron homeostasis. This clearly highlighted the importance of eventually testing several growth conditions and pinpointed the power of proteomics helping building mechanistic hypotheses. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-09_03:52:25.037.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055248 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hélène Diemer |
| SpeciesList | scientific name: Bacillus subtilis; NCBI TaxID: NEWT:1423; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-27 06:54:33 | ID requested | |
| ⏵ 1 | 2026-02-09 03:52:25 | announced | |
Publication List
Keyword List
| submitter keyword: swarming, nanoceria, shotgun proteomic, Bacillus subtilis, nanoparticles,Pellicle biofilm, LC MSMS |
Contact List
| Sarah CIANFERANI |
|---|
| contact affiliation | Laboratoire de Spectrometrie de Masse BioOrganique (LSMBO), Universite de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg, France |
| contact email | sarah.cianferani@unistra.fr |
| lab head | |
| Hélène Diemer |
|---|
| contact affiliation | LSMBO |
| contact email | hdiemer@unistra.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD055248 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055248
- Label: PRIDE project
- Name: Comparison of Cerium nanoparticles toxicity against Bacillus subtilis grown in two different types of biofilm
