PXD055236

PXD055236 is an original dataset announced via ProteomeXchange.

Title	Histone lactylation: a new epigenetic mark in the malaria parasite Plasmodium
Description	In the malaria parasite Plasmodium falciparum, epigenetic modifications such as acetylation and methylation play important roles in parasite biology and virulence. Here, we characterised a new epigenetic mark, histone lactylation, recently discovered in humans and also present in Plasmodium. It was found in two human malaria parasites, P. falciparum and the zoonotic macaque parasite P. knowlesi, and was also found in vivo in two rodent malaria models. Histones were lactylated rapidly in response to elevated lactate levels, either exogenously added or endogenously generated by the parasite's own metabolism, and they were rapidly delactylated when lactate levels fell. Thus, this epigenetic mark is well-placed to act as a metabolic sensor, since severe falciparum malaria characteristically leads to hyperlactataemia in infected patients. Mass spectrometry showed that lysines on several parasite histones could be lactylated, and that this was accompanied by lactylation of many non-histone chromatin proteins. Histone lactylation was less abundant and less inducible in P. knowlesi than in P. falciparum, suggesting that P. falciparum may have evolved particular epigenetic responses to this characteristic feature of its pathology. Finally, in the rodent model P. yoelii, hyperlactataemia correlated with parasite transcriptomic programmes that suggested metabolic 'dormancy'.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_03:06:49.686.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055236
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Catherine Merrick
SpeciesList	scientific name: Plasmodium falciparum NF54; NCBI TaxID: NEWT:5843; scientific name: Plasmodium knowlesi strain H; NCBI TaxID: NEWT:5851;
ModificationList	carbamoylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-27 05:49:22	ID requested
⏵ 1	2025-12-15 03:06:50	announced

10.6019/PXD055236;

submitter keyword: lactylation, histone, mass spec, malaria,Epigenetics

Catherine J. Merrick
contact affiliation	Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
contact email	cjm48@cam.ac.uk
lab head
Catherine Merrick
contact affiliation	Cambridge University
contact email	cjm48@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055236
     1. Label: PRIDE project
     2. Name: Histone lactylation: a new epigenetic mark in the malaria parasite Plasmodium