PXD055233 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DNMT3L safeguards the epigenome and developmental potential of naive human pluripotent stem cells and blastoids |
| Description | DNA methylation is essential for mammalian development. Studies of the catalytically inactive DNA methyltransferase Dnmt3l have mainly been limited to mice where Dnmt3l was shown to be essential for fertility, de novo DNA methylation in early embryos and maintenance of DNA methylation patterns in mouse pluripotent stem cells. Recent work revealed that DNMT3L is the most highly enriched chromatin factor in naive human pluripotent stem cells (hPSCs) compared with primed hPSCs. Stem cell-based human embryo models and naive hPSCs provide a unique opportunity to functionally dissect human developmental mechanisms. Here we use naive hPSCs and human blastoids, a 3D human blastocyst model, to investigate the functional impact of DNMT3L on human naive pluripotency and preimplantation development. We demonstrate an essential role of DNMT3L in trophectoderm cell fate induction, maintenance of DNA methylation patterns and chromatin states in naive hPSCs, and blastoid lineage identity. We further show that DNMT3L promotes reprogramming to naive pluripotency, and has integrated a hominoid-specific naive human pluripotency gene regulatory program involving evolutionary recent transposable elements. Therefore, DNMT3L plays conserved and hominoid-specific functions in human naive pluripotency and pre-implantation development. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_14:47:01.498.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zicong Liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-27 05:36:12 | ID requested | |
| ⏵ 1 | 2026-08-25 14:47:02 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Keywords: DNMT3L |
| Human Naïve ESCs |
| DNA methylation |
| hPTMs |
| blastoids |
| Epigenestis |
Contact List
| Hendrik Marks |
|---|
| contact affiliation | Radboud Universiteit Nijmegen |
| contact email | hendrik.marks@ru.nl |
| lab head | |
| Zicong Liu |
|---|
| contact affiliation | Radboud Universiteit Nijmegen |
| contact email | zicong.liu@ru.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055233
- Label: PRIDE project
- Name: DNMT3L safeguards the epigenome and developmental potential of naive human pluripotent stem cells and blastoids
