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PXD055214

PXD055214 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn integrative analysis of cell-specific transcriptomics and nuclear proteomics of sleep-deprived mouse cerebral cortex
DescriptionSleep regulation follows a homeostatic pattern. The mammalian cerebral cortex is the repository of homeostatic sleep drive and neurons and astrocytes of the cortex are principal responders of sleep need. The molecular mechanisms by which these two cell types respond to sleep loss are not yet clearly understood. By combining cell-type specific transcriptomics and nuclear proteomics we investigated how sleep loss affects the cellular composition and molecular profiles of these two cell types in a focused approach. The results indicate that sleep deprivation regulates gene expression and nuclear protein abundance in a cell-type-specific manner. Our integrated multi-omics analysis suggests that this distinction arises because neurons and astrocytes employ different gene regulatory strategies under accumulated sleep pressure. These findings provide a comprehensive view of the effects of sleep deprivation on gene regulation in neurons and astrocytes.
HostingRepositoryPRIDE
AnnounceDate2025-05-30
AnnouncementXMLSubmission_2025-05-30_09:44:55.250.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD055214
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPawan Jha
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-26 13:10:21ID requested
12025-05-30 09:44:55announced
Publication List
10.6019/PXD055214;
Keyword List
submitter keyword: Sleep deprivation, Neuron, Gene regulation,Astrocyte, Nuclear proteomics
Contact List
Akhilesh B. Reddy
contact affiliationDepartment of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
contact emailareddy@cantab.ne
lab head
Pawan Jha
contact affiliationDepartment of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
contact emailkj.pawan@gmail.com
dataset submitter
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Dataset FTP location
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