PXD055214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An integrative analysis of cell-specific transcriptomics and nuclear proteomics of sleep-deprived mouse cerebral cortex |
Description | Sleep regulation follows a homeostatic pattern. The mammalian cerebral cortex is the repository of homeostatic sleep drive and neurons and astrocytes of the cortex are principal responders of sleep need. The molecular mechanisms by which these two cell types respond to sleep loss are not yet clearly understood. By combining cell-type specific transcriptomics and nuclear proteomics we investigated how sleep loss affects the cellular composition and molecular profiles of these two cell types in a focused approach. The results indicate that sleep deprivation regulates gene expression and nuclear protein abundance in a cell-type-specific manner. Our integrated multi-omics analysis suggests that this distinction arises because neurons and astrocytes employ different gene regulatory strategies under accumulated sleep pressure. These findings provide a comprehensive view of the effects of sleep deprivation on gene regulation in neurons and astrocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-30 |
AnnouncementXML | Submission_2025-05-30_09:44:55.250.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055214 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Pawan Jha |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-26 13:10:21 | ID requested | |
⏵ 1 | 2025-05-30 09:44:55 | announced | |
Publication List
Keyword List
submitter keyword: Sleep deprivation, Neuron, Gene regulation,Astrocyte, Nuclear proteomics |
Contact List
Akhilesh B. Reddy |
contact affiliation | Department of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA |
contact email | areddy@cantab.ne |
lab head | |
Pawan Jha |
contact affiliation | Department of Systems Pharmacology & Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA |
contact email | kj.pawan@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055214
- Label: PRIDE project
- Name: An integrative analysis of cell-specific transcriptomics and nuclear proteomics of sleep-deprived mouse cerebral cortex