PXD055200

PXD055200 is an original dataset announced via ProteomeXchange.

Title	The phosphoproteome of recombinant 2G12 and 353/11 mAb producing CHO-K1 cell lines
Description	In this study, we conducted a phosphoproteomic analysis of two cell lines with differing monoclonal antibody (mAb) production levels using label-free quantification mass spectrometry (LFQ-MS). The high-producing cell line, 353/11, was compared to the lower-producing 2G12 cell line. Phosphoproteomic samples were collected on day six of cell culture. To ensure synchronized sampling, all samples were harvested four hours after medium exchange. Additionally, 353/11 cells were separately cultivated and treated in parallel with 1 µg/ml tunicamycin (TM) for four hours to induce mild endoplasmic reticulum (ER) stress.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_01:35:25.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eldi Sulaj
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-26 05:33:25	ID requested
⏵ 1	2025-05-07 01:35:26	announced

10.3390/proteomes13010009;

Sulaj E, Sandell FL, Schwaigerlehner L, Marzban G, Dohm JC, Kunert R, Systems Biology of Recombinant 2G12 and 353/11 mAb Production in CHO-K1 Cell Lines at Phosphoproteome Level. Proteomes, 13(1):(2025) [pubmed]

submitter keyword: tunicamycin,Chinese hamster ovary, phosphoproteome, mass spectrometry, LFQ

Gorji Marzban
contact affiliation	Department of Biotechnology,Institute of Bioprocess Science and Engineering (IBSE), BOKU University
contact email	gorji.marzban@boku.ac.at
lab head
Eldi Sulaj
contact affiliation	BOKU University, Vienna
contact email	eldi.sulaj@boku.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055200

PRIDE project URI

• PRIDE
  1. PXD055200
     1. Label: PRIDE project
     2. Name: The phosphoproteome of recombinant 2G12 and 353/11 mAb producing CHO-K1 cell lines