PXD055195

PXD055195 is an original dataset announced via ProteomeXchange.

Title	The autophagy receptor SQSTM1/p62 is a restriction factor of HCMV infection
Description	Background: Intrinsic defence mechanisms are pivotal host strategies to restrict viruses already at early stages of their infection. Here we addressed the question how the autophagy receptor sequestome 1 (SQSTM1/p62, herein termed p62) interfered with human cytomegalovirus (HCMV) infection. (2) Methods: CRISPR/Cas9- mediated genome editing, mass spectrometry and expression of p62 phosphovariants from recombinant HCMVs were used to address the role of p62 during infection. (3) Results: Knockout of p62 led to enhanced HCMV progeny release. Mass spectrometry revealed an interaction of p62 with cellular proteins required for nucleo-cytoplasmic transport. Phosphoproteomics further revealed that p62 is hyperphosphorylated at position S272 in HCMV-infected cells. Phosphorylated p62 showed enhanced nuclear retention, concordant with enhanced interaction with viral proteins relevant for genome replication and nuclear capsid egress. This modification led to reduced HCMV progeny release, compared to a unphosphorylated version of p62. (4) Conclusions: p62 is a restriction factor for HCMV replication The activity of the receptor appears to be regulated by phosphorylation at position S272, leading to enhanced nuclear localization, viral protein degradation and impairment of progeny production.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_01:33:03.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uxia Gestal Mato
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-26 03:40:58	ID requested
⏵ 1	2025-05-07 01:33:04	announced

Kr, ä, mer N, Mato UG, Krauter S, B, ü, scher N, Afifi A, Herhaus L, Florin L, Plachter B, Zimmermann C, /p62 Is a Restriction Factor of HCMV Infection. Viruses, 16(9):(2024) [pubmed]

10.3390/v16091440;

submitter keyword: human cytomegalovirus

autophagy receptor

SQSTM1/p62

optineurin

host cell defence

Lina Herhaus
contact affiliation	Team Leader Immune Signaling Institute of Biochemistry 2 Goethe University Medical School
contact email	herhaus@em.uni-frankfurt.de
lab head
Uxia Gestal Mato
contact affiliation	Institut für Biochemie II - Goethe Universität Frankfurt
contact email	gestalmato@em.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055195
     1. Label: PRIDE project
     2. Name: The autophagy receptor SQSTM1/p62 is a restriction factor of HCMV infection