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PXD055194

PXD055194 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe autophagy receptor SQSTM1/p62 is a restriction factor of HCMV infection
Description(1) Background: Intrinsic defence mechanisms are pivotal host strategies to restrict viruses already at early stages of their infection. Here we addressed the question how the autophagy receptor sequestome 1 (SQSTM1/p62, herein termed p62) interfered with human cytomegalovirus (HCMV) infection. (2) Methods: CRISPR/Cas9- mediated genome editing, mass spectrometry and expression of p62 phosphovariants from recombinant HCMVs were used to address the role of p62 during infection. (3) Results: Knockout of p62 led to enhanced HCMV progeny release. Mass spectrometry revealed an interaction of p62 with cellular proteins required for nucleo-cytoplasmic transport. Phosphoproteomics further revealed that p62 is hyperphosphorylated at position S272 in HCMV-infected cells. Phosphorylated p62 showed enhanced nuclear retention, concordant with enhanced interaction with viral proteins relevant for genome replication and nuclear capsid egress. This modification led to reduced HCMV progeny release, compared to a unphosphorylated version of p62. (4) Conclusions: p62 is a restriction factor for HCMV replication The activity of the receptor appears to be regulated by phosphorylation at position S272, leading to enhanced nuclear localization, viral protein degradation and impairment of progeny production.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_01:35:42.280.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUxia Gestal Mato
SpeciesList scientific name: Cytomegalovirus; NCBI TaxID: 10358; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-26 03:25:45ID requested
12025-05-07 01:35:43announced
Publication List
Kr, ä, mer N, Mato UG, Krauter S, B, ü, scher N, Afifi A, Herhaus L, Florin L, Plachter B, Zimmermann C, /p62 Is a Restriction Factor of HCMV Infection. Viruses, 16(9):(2024) [pubmed]
10.3390/v16091440;
Keyword List
submitter keyword: human cytomegalovirus
autophagy receptor
SQSTM1/p62
optineurin
host cell defence
Contact List
Lina Herhaus
contact affiliationTeam Leader Immune Signaling Institute of Biochemistry 2 Goethe University Medical School
contact emailherhaus@em.uni-frankfurt.de
lab head
Uxia Gestal Mato
contact affiliationInstitut für Biochemie II - Goethe Universität Frankfurt
contact emailgestalmato@em.uni-frankfurt.de
dataset submitter
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