PXD055180

PXD055180 is an original dataset announced via ProteomeXchange.

Title	Spectral library-enhanced label-free proteome profiling highlights the critical role of the right atrium in persistent atrial fibrillation
Description	Pulmonary veins (PVs) in the left atrium (LA) are a critical site for the initiation of atrial fibrillation (AF). However, the persistent AF (persAF) extends beyond the LA, encompassing both atrial chambers with widespread AF drivers that further promote AF. However, PV ablation remains a cornerstone of AF management. Considering high post-ablation AF recurrence rates in persAF patients, we hypothesized that the right atrium (RA) may play a significant role in the persistence of AF. In this study, we established a comprehensive reference spectral library for the analysis of the human cardiac proteome by data-independent acquisition mass spectrometry (DIA-MS) using material from donor hearts. Application of this library enabled time-efficient and robust analysis of the persAF proteome using RA appendages from patients. Our findings reveal the presence of canonical AF markers and processes within the RA in persAF. Additionally, we observed that the RA mirrors the LA at the proteomic level, losing RA-specific and gaining LA-specific signature proteins in persAF. We conclude that the RA plays a pivotal role in sustaining persAF, underscoring its importance in the clinical management of AF.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_11:16:14.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-25 11:45:38	ID requested
⏵ 1	2026-06-12 11:16:14	announced

10.1093/CVR/CVAG076;

submitter keyword: cardiac,atrial fibrillation, mass spectrometry, proteome, right ventricle

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Chemistry, Universitry Medical Center Göttingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD055180

PRIDE project URI

• PRIDE
  1. PXD055180
     1. Label: PRIDE project
     2. Name: Spectral library-enhanced label-free proteome profiling highlights the critical role of the right atrium in persistent atrial fibrillation