PXD055175

PXD055175 is an original dataset announced via ProteomeXchange.

Title	Elucidating the Roles of MCM7 and SHCBP1 in Liver Fibrosis through LC-MS Analysis
Description	Liver fibrosis, characterized by the excessive deposition of extracellular matrix, presents a significant challenge due to its intricately complex and poorly understood mechanisms. In this study, we aimed to elucidate the roles of Minichromosome Maintenance Complex Component 7 (MCM7) and SHC SH2-Domain Binding Protein 1 (SHCBP1) in liver fibrosis, focusing on their regulatory networks and interactions. Stable hepatocyte cell lines with overexpression of MCM7 and SHCBP1 were constructed. Through targeted LC-MS analysis of MCM7, significant changes were observed in the protein interaction profiles, . MCM7 was found to interact with SHCBP1, which was further analyzed using LC-MS to detail its role in binding to RACGAP1. This interaction activates the SHCBP1-RACGAP1-STAT3 signaling axis, leading to increased IL11 expression. Elevated IL11 levels contribute to the activation of hepatic stellate cells (HSCs) and the progression of liver fibrosis. Our findings demonstrate that through mass spectrometry, MCM7 is identified as a crucial regulatory factor in liver fibrosis, promoting IL11 expression via the SHCBP1-RACGAP1-STAT3 signaling pathway. Both MCM7 and SHCBP1 involvement in driving HSC activation and fibrosis progression. Overall, our study highlights a mechanistic pathway where MCM7 and SHCBP1 collaborate to regulate IL11 expression, thereby influencing the progression of liver fibrosis. These insights emphasize the critical regulatory roles of MCM7 and SHCBP1, offering potential therapeutic targets for combating fibrotic liver diseases.
HostingRepository	PRIDE
AnnounceDate	2025-08-09
AnnouncementXML	Submission_2025-08-08_21:14:49.704.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	rui zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-25 08:23:10	ID requested
⏵ 1	2025-08-08 21:14:50	announced

10.1038/S41419-025-07937-X;

submitter keyword: ,Liver Fibrosis, LC-MS, MCM7, IL11, SHCBP1, Protein Interaction

ruizhu lab
contact affiliation	Wuhan University
contact email	chengwangi@whu.edu.cn
lab head
rui zhou
contact affiliation	wuhan university
contact email	ruizhou@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD055175

PRIDE project URI

• PRIDE
  1. PXD055175
     1. Label: PRIDE project
     2. Name: Elucidating the Roles of MCM7 and SHCBP1 in Liver Fibrosis through LC-MS Analysis