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PXD055173

PXD055173 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleiDIA-QC: AI-empowered Data-Independent Acquisition Mass Spectrometry-based Quality Control
DescriptionQuality control (QC) in mass spectrometry (MS)-based proteomics is mainly based on data-dependent acquisition (DDA) analysis of standard samples. Here, we collected 2638 files acquired by data independent acquisition (DIA) and paired DDA files from mouse liver digests using 21 mass spectrometers across nine laboratories over 31 months. Our data demonstrated that DIA-based LC-MS/MS-related consensus QC metrics exhibit higher sensitivity compared to DDA-based QC metrics in detecting changes in LC-MS status. We then optimized 15 metrics and invited 21 experts to manually assess the quality of 2638 DIA files based on those metrics. Based on the annotation results, we developed an AI model for DIA-based QC in the training set of 2110 DIA files. This model predicted the liquid chromatography (LC) performance with an AUC of 0.91 and the MS performance with an AUC of 0.97 in an independent validation dataset (n = 528). Finally, we developed an offline software called iDIA-QC for convenient adoption of this methodology for LC-MS QC.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_01:48:30.243.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitter欢 高
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-24 21:33:59ID requested
12025-05-07 01:48:32announced
Publication List
Gao H, Zhu Y, Wang D, Nie Z, Wang H, Wang G, Liang S, Xie Y, Sun Y, Jiang W, Dong Z, Qian L, Wang X, Liang M, Chen M, Fang H, Zeng Q, Tian J, Sun Z, Xue J, Li S, Chen C, Liu X, Lyu X, Guo Z, Qi Y, Wu R, Du X, Tong T, Kong F, Han L, Wang M, Zhao Y, Dai X, He F, Guo T, iDIA-QC: AI-empowered data-independent acquisition mass spectrometry-based quality control. Nat Commun, 16(1):892(2025) [pubmed]
10.1038/s41467-024-54871-1;
Keyword List
submitter keyword: machine learning, DIA,quality control, SWATH, mouse liver, diaPASEF
Contact List
Tiannan Guo
contact affiliationWestlake University
contact emailguotiannan@westlake.edu.cn
lab head
欢 高
contact affiliationWestlake University
contact emailgaohuanhuan@westlake.edu.cn
dataset submitter
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Dataset FTP location
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