PXD055166

PXD055166 is an original dataset announced via ProteomeXchange.

Title	Identification of PP1-fusion phosphatase substrates
Description	This experiments was designed to identify substrates of holophosphatase complexes of PP1 and its specificity-determining regulator proteins. We generated fusions of Flag-tagged Protein Phosphatase 1 fusions with Phactr1-4, Neurabin, Spinophilin, PPPR15A, PPP1R15B, and PNUTS, in each case including known protein interaction domains immediately C-terminal to the PP1-binding sequences. Each fusion protein was stably expressed in 293 Flp-In TRex cells using a tetracycline inducible vector. To investigate the substrate specificities of the PP1-fusion proteins, we performed Tandem-Mass-Tag (TMT) phosphoproteomics. Fractionated peptides were measured in both MS2 and MS3 modes for maximal identification and quantification. Expression of the PP1-fusions revealed numerous phosphorylation sites that were specifically depleted compared with cells expressing PP1 alone.
HostingRepository	PRIDE
AnnounceDate	2025-05-28
AnnouncementXML	Submission_2025-05-28_02:15:23.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-24 06:04:40	ID requested
⏵ 1	2025-05-28 02:15:24	announced

Dataset with its publication pending

submitter keyword: Spinophilin, TMT,phosphatase, phosphoproteomics, Phactr1, PP1

Richard Treisman
contact affiliation	The Francis Crick Institute, London, UK
contact email	richard.treisman@crick.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055166
     1. Label: PRIDE project
     2. Name: Identification of PP1-fusion phosphatase substrates