PXD055153

PXD055153 is an original dataset announced via ProteomeXchange.

Title	Restoration of growth and isoprenol production in dual carbon source media by an acetate-sensitive Pseudomonas putida strain
Description	Lignocellulosic biomass is an abundant and renewable resource that can be leveraged for the production of a diverse array of platform chemicals, including next generation biofuels such as isoprenol. One of the main obstacles for the efficient use of lignocellulosic biomass as a substrate are inhibitors formed during pretreatment that pose challenging for the development of efficient and cost-effective microbial processes. Acetate is a growth-inhibitory organic acid derived from O-acetylated hemicellulose and has high concentrations in many lignocellulosic biomass hydrolysates. In this work, we adapted Pseudomonas putida as an acetate-tolerant chassis for bioproduction with increasing concentrations of both the heterologous product (isioprenol) and acetate. Using this approach, we restored cell growth when acetate is provided as the sole carbon source. Our results show we possibly have rerouted the entry of acetate into a different step for entry into the TCA. We demonstrate the use of rational engineering and ALE to synergistically enable the development of robust production strains for the bioconversion processes with this abundant alternative substrate.
HostingRepository	PRIDE
AnnounceDate	2025-02-27
AnnouncementXML	Submission_2025-02-27_13:02:33.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 14:28:04	ID requested
⏵ 1	2025-02-27 13:02:34	announced

10.1128/AEM.02123-24;

submitter keyword: Pseudomonas putida, Bioproduction, Acetate-tolerant,Isoprenol

Christopher J. Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055153
     1. Label: PRIDE project
     2. Name: Restoration of growth and isoprenol production in dual carbon source media by an acetate-sensitive Pseudomonas putida strain