PXD055142

PXD055142 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Differential expression of proteins due to Cobalt(II) Chloride induced pseudohypoxia in Jurkat cells
Description	To demonstrate the mechanisms underlying Cobalt(II) Chloride driven pseudohypoxia, proteomic dysregulations were studied in a human T-cell Lymphoma cell line (Jurkat). This proteomics data generated the abundance ratios of proteins in CoCl2 treated versus untreated Jurkat cells. Concisely, Jurkat cells were treated with CoCl2 (50uM) in complete RPMI media. Tryptic digested and desalted peptide samples were injected in Thermoscientific Q-Exactive Plus instruments through EasyNLC HPLC autosampler. The instruments were set to MS1 resolution of 70000 and MS2 resolution of 17500. The acquisition experiments were optimized to run on 2 hours gradients. The MS spectra were analyzed using the Thermoscientific mass informatics platform Proteome discoverer version 2.2. The common workflows for discovery proteomics were used with Mascot and SequestHT. This dataset assisted in identifying the pseudohypoxia-driven changes in lymphoma progression and revealed many druggable targets in the context of metastasis in T-cell malignanices.
HostingRepository	MassIVE
AnnounceDate	2025-05-20
AnnouncementXML	Submission_2025-05-20_02:21:14.211.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ditipriya Mukherjee
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 09:16:01	ID requested
⏵ 1	2025-05-20 02:21:14	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Jurkat, Pseudohypoxia, Cobalt chloride

submitter keyword: Jurkat, Pseudohypoxia, Cobalt chloride

Contact List

Rahul Purwar
contact affiliation	IIT Bombay
contact email	purwarrahul@iitb.ac.in
lab head
Ditipriya Mukherjee
contact affiliation	Indian Institute of Technology Bombay
contact email	195300019@iitb.ac.in
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095681/