PXD055136

PXD055136 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the fish pathogen Vibrio ordalii strain Vo-LM-18 and its outer membrane vesicles
Description	Vibrio ordalii is the causative agent of atypical vibriosis in salmonids cultured in Chile. While extensive research has provided valuable insights into V. ordalii through phenotypic, antigenic, and genetic typing, as well as various virulence mechanisms (e.g., capsular material, iron-uptake systems, and outer membrane vesicles [OMVs]), proteomic characterization remains largely unexplored. This study aimed to advance the proteomic knowledge of Chilean V. ordalii Vo-LM-18 and its OMVs, which have been extensively characterized in several studies and have demonstrated their virulence. Using Nano-UHPLC-LC-MS/MS, we identified 2,242 proteins in the bacterium and 1,755 proteins in its OMVs. Among these, 644 unique proteins were detected in V. ordalii Vo-LM-18, 156 unique proteins were found in its OMVs, and 1,596 proteins were shared between both components. bioinformatics compartment prediction (PSORT algorithm) revealed that the major categories in the OMVs were similar to those in the bacteria (i.e., cytoplasmic and cytoplasmic membrane proteins), though with different proportions. Functional annotation with Gene Ontology identified 37 biological pathways in V. ordalii Vo-LM-18 and 28 in its OMVs. Proteins associated with transport, transcription, and virulence were predominant in both the bacteria and the vesicles. Comparison between the strain Vo-LM-18 and its OMVs showed evident differences in protein expression, with OMVs expressing a higher number of virulence-associated proteins, including those related to iron- and heme-uptake mechanisms. Notable pathways in the bacteria included flagellum assembly, heme group-associated proteins, and protein biosynthesis. This proteomic analysis also represents the first detection of RTX toxin in a V. ordalii strain (Vo-LM-18) and its vesicles. Our results highlight the crucial role of OMVs in the pathogenesis and adaptation of V. ordalii, suggesting their potential as diagnostic biomarkers and therapeutic targets for bacterial infections.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-14_11:00:32.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillermo Nourdin
SpeciesList	scientific name: Vibrio ordalii; NCBI TaxID: NEWT:28174;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-23 08:21:34	ID requested
⏵ 1	2026-05-14 11:00:33	announced

10.3390/ANI14243598;

submitter keyword: salmonids,Vibriosis, OMVs., proteome

Ruben Avendaño-Herrera
contact affiliation	Universidad Andrés Bello, Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias de la Vida, Viña del Mar, Chile
contact email	ravendano@unab.cl
lab head
Guillermo Nourdin
contact affiliation	MELISA Institute
contact email	gnourdin@melisainstitute.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055136
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the fish pathogen Vibrio ordalii strain Vo-LM-18 and its outer membrane vesicles