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PXD055109

PXD055109 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCHAMP1 complex directs heterochromatin assembly and promotes homology-directed DNA repair
DescriptionThe CHAMP1 complex, a little-known but highly conserved protein complex consisting of CHAMP1, POGZ, and HP1, is enriched in heterochromatin though its cellular function in these regions of the genome remain unknown. Here we show that the CHAMP complex promotes heterochromatin assembly at multiple chromosomal sites, including centromeres and telomeres, and promotes homology-directed repair (HDR) of DNA double strand breaks (DSBs) in these regions. The CHAMP1 complex is also required for heterochromatin assembly and DSB repair in highly-specialized chromosomal regions, such as the highly-compacted telomeres of ALT (Alternative Lengthening of Telomeres) positive tumor cells. Moreover, the CHAMP1 complex binds and recruits the writer methyltransferase SETDB1 to heterochromatin regions of the genome and is required for efficient DSB repair at these sites. Importantly, peripheral blood lymphocytes from individuals with CHAMP1 syndrome, an inherited neurologic disorder resulting from heterozygous mutations in CHAMP1, also exhibit defective heterochromatin clustering and defective repair of local DSBs, suggesting that a defect in DNA repair underlies this syndrome. Taken together, the CHAMP1 complex has a novel role in heterochromatin assembly and the enhancement of HDR in heterochromatin.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_01:05:21.057.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHuy Nguyen
SpeciesList scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-23 00:05:55ID requested
12025-05-07 01:05:22announced
Publication List
10.1038/s41467-025-56834-6;
Li F, Zhang T, Syed A, Elbakry A, Holmer N, Nguyen H, Mukkavalli S, Greenberg RA, D'Andrea AD, CHAMP1 complex directs heterochromatin assembly and promotes homology-directed DNA repair. Nat Commun, 16(1):1714(2025) [pubmed]
Keyword List
submitter keyword: Human
Contact List
Alan D'Andrea
contact affiliationDepartment of Radiation Oncology Dana-Farber Cancer Institute USA
contact emailAlan_Dandrea@dfci.harvard.edu
lab head
Huy Nguyen
contact affiliationDana-Farber Cancer Institute
contact emailhuy_nguyen@dfci.harvard.edu
dataset submitter
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Dataset FTP location
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