PXD055096
PXD055096 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ultra-Photosensitization of Fluorescent Protein Chromophores towards Photocatalytic Proximity Labeling and Proteomic Profiling |
Description | Due to relatively lower reactive oxygen species (ROS) quantum yield, photosensitizing fluorescent proteins (FP) (e.g. KillerRed) have been shown not suitable for photo-catalytic protein proximity labeling and downstream proteomic profiling applications. To acquire such functions, we engineer FP chromophores in a 12×12 combinatorial matrix of synthetic analogues, yielding maximally over 1000-fold enhancement of reactive oxygen species (ROS) production. We show the ROS yield is correlated to the directionality of “push-pull” effect of FP chromophores. After conjugation to HaloTag, the ultra-photosensitized FP mimics (namely upsFP) herein realizes photo-catalytic protein proximity labeling using nucleophilic amino substrates. A set of photochemical characterizations, theoretical calculation, and tandem mass spectrometry collectively reveals radical mediated labeling with expanded reactivity towards protein residues via type I photosensitization pathway. Finally, we showcase a proteomic profiling application by using the upsFP tag to resolve the dynamic interactome variations upon TAR DNA-binding protein 43 (TDP43) phase separation and suborganellar translocation. Together, this work demonstrates 3 orders of magnitude ultra- photosensitization enables photocatalytic proximity labeling and profiling functions for biomimetic fluorescent proteins. |
HostingRepository | iProX |
AnnounceDate | 2024-08-23 |
AnnouncementXML | Submission_2024-08-22_20:16:07.097.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nan Zhao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-08-22 20:15:35 | ID requested | |
⏵ 1 | 2024-08-22 20:16:07 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: fluorescent protein, proximity labeling, photosensitization, proteomics, phase separation |
Contact List
Lihua Zhang | |
---|---|
contact affiliation | Dalian Institute of Chemical Physics |
contact email | lihuazhang@dicp.ac.cn |
lab head | |
Nan Zhao | |
contact affiliation | Dalian Institute of Chemical Physics |
contact email | zhaonan@dicp.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |