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PXD055096

PXD055096 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUltra-Photosensitization of Fluorescent Protein Chromophores towards Photocatalytic Proximity Labeling and Proteomic Profiling
DescriptionDue to relatively lower reactive oxygen species (ROS) quantum yield, photosensitizing fluorescent proteins (FP) (e.g. KillerRed) have been shown not suitable for photo-catalytic protein proximity labeling and downstream proteomic profiling applications. To acquire such functions, we engineer FP chromophores in a 12×12 combinatorial matrix of synthetic analogues, yielding maximally over 1000-fold enhancement of reactive oxygen species (ROS) production. We show the ROS yield is correlated to the directionality of “push-pull” effect of FP chromophores. After conjugation to HaloTag, the ultra-photosensitized FP mimics (namely upsFP) herein realizes photo-catalytic protein proximity labeling using nucleophilic amino substrates. A set of photochemical characterizations, theoretical calculation, and tandem mass spectrometry collectively reveals radical mediated labeling with expanded reactivity towards protein residues via type I photosensitization pathway. Finally, we showcase a proteomic profiling application by using the upsFP tag to resolve the dynamic interactome variations upon TAR DNA-binding protein 43 (TDP43) phase separation and suborganellar translocation. Together, this work demonstrates 3 orders of magnitude ultra- photosensitization enables photocatalytic proximity labeling and profiling functions for biomimetic fluorescent proteins.
HostingRepositoryiProX
AnnounceDate2024-08-23
AnnouncementXMLSubmission_2024-08-22_20:16:07.097.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNan Zhao
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-22 20:15:35ID requested
12024-08-22 20:16:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: fluorescent protein, proximity labeling, photosensitization, proteomics, phase separation
Contact List
Lihua Zhang
contact affiliationDalian Institute of Chemical Physics
contact emaillihuazhang@dicp.ac.cn
lab head
Nan Zhao
contact affiliationDalian Institute of Chemical Physics
contact emailzhaonan@dicp.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI