PXD055093 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics |
| Description | We combine the membrane mimetic (MM) Peptidisc with thermal proteome profiling (TPP) to screen membrane proteomes in detergent-free environments. Using whole mouse liver, we demonstrate the stabilization of integral membrane proteins (IMPs), specifically ATP-binding cassette transporters, through interactions with ATP and orthovanadate. Additionally, we assess the downstream effects of ATP hydrolysis products on IMP stability, such as that of GPCR purinergic receptors, and validate novel interactions with AlphaFold3 |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-17 |
| AnnouncementXML | Submission_2025-11-16_20:13:55.152.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rupinder Jandu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-22 16:55:05 | ID requested | |
| ⏵ 1 | 2025-11-16 20:13:57 | announced | |
Publication List
| Jandu RS, Bhattacharya A, Antony F, Al-Seragi M, Aoki H, Babu M, Duong van Hoa F, Membrane-mimetic thermal proteome profiling (MM-TPP) toward mapping membrane protein-ligand dynamic interactions. Elife, 14():(2025) [pubmed] |
| 10.7554/elife.104549; |
Keyword List
| submitter keyword: Membrane Mimetic,Mouse Liver, Thermal Proteome Profiling |
Contact List
| Franck Duong van Hoa |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada. |
| contact email | fduong@mail.ubc.ca |
| lab head | |
| Rupinder Jandu |
|---|
| contact affiliation | University of British Columbia |
| contact email | rupjandu@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD055093 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055093
- Label: PRIDE project
- Name: Membrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
