PXD055092

PXD055092 is an original dataset announced via ProteomeXchange.

Title	The iron-regulated sRNA IsrR is involved in the display of full Staphylococcus aureus virulence in part via SaeRS activation
Description	The Gram-positive pathogenic bacterium Staphylococcus aureus permanently colonizes the nasal mucosa in about 30% of the healthy population. However, as an opportunistic pathogen, S. aureus can also cause a variety of diseases, the treatment of which is becoming increasingly difficult due to antibiotic resistance. Adaptation to changing environmental conditions during colonization and infection of the host requires precise regulation of the expression of genes for virulence factors and metabolic functions. The host conditions essentially include low iron availability, with the majority of iron in the human organism being present intracellularly in the form of haemoglobin. Therefore, S. aureus has various adaptive mechanisms, in particular iron uptake systems, which are regulated by the global iron uptake regulator Fur (ferric-uptake regulator). Iron deficiency conditions lead to the derepression of Fur-dependent genes. In many bacteria, these genes also include regulatory RNAs (small regulatory RNAs, sRNAs), which mediate an iron-sparing response by inhibiting the synthesis of non-essential iron-containing proteins. A Fur-dependent sRNA (S596) was identified in a transcriptome analysis of S. aureus. In a study by Coronel-Tellez et al. (2022) it was shown that S596/IsrR (“iron sparing response regulator”) is necessary for the virulence of S. aureus. In the presented secretome analysis, the S. aureus HG001 wild type (WT) was compared with an isogenic isrR mutant (ΔisrR) under iron-limiting conditions and a constitutively isrR-expressing strain (HG001 pJLisrR) with an empty vector control (HG001 pJLctrl) under iron-rich conditions as well as corresponding sae mutant (Δsae) strains. Culture supernatants were collected in the exponential and stationary growth phases and extracellular proteins were subsequently analyzed by mass spectrometry. Statistical data analysis was performed to identify proteins with significantly altered amounts between the respective strains. We demonstrate that IsrR positively influences the protein levels of numerous virulence factors which in particular belong to the Sae regulon.
HostingRepository	PRIDE
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-11_02:47:46.625.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Larissa Busch
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 16:32:32	ID requested
⏵ 1	2025-04-11 02:47:47	announced

Dataset with its publication pending

submitter keyword: SaeRS, virulence,Staphylococcus aureus, secretome, IsrR

Uwe Völker
contact affiliation	University Medicine Grefifswald, Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics
contact email	voelker@uni-greifswald.de
lab head
Larissa Busch
contact affiliation	Department of Functional Genomics, University Medicine Greifswald
contact email	larissa.busch@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055092
     1. Label: PRIDE project
     2. Name: The iron-regulated sRNA IsrR is involved in the display of full Staphylococcus aureus virulence in part via SaeRS activation