PXD055090 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment |
Description | Epilepsy is characterized by hypersynchronous neuronal discharges, which are associated with an increased cerebral metabolic rate of oxygen and ATP demand. Uncontrolled seizure activity (status epilepticus) results in mitochondrial exhaustion and ATP depletion, which potentially generate energy mismatch and neuronal loss. Many cells can adapt to increased energy demand by increasing metabolic capacities. However, acute metabolic adaptation during epileptic activity and its relationship to chronic epilepsy remains poorly understood. We elicited seizure-like events (SLEs) in an in vitro model of status epilepticus for eight hours. Electrophysiological recording and tissue oxygen partial pressure recordings were performed. After eight hours of ongoing SLEs, we used proteomics-based kinetic modeling to evaluate changes in metabolic capacities. We compared our findings regarding acute metabolic adaptation to published proteomic and transcriptomic data from chronic epilepsy patients. Epileptic tissue acutely responded to uninterrupted SLEs by upregulating ATP production capacity. This was achieved by a coordinated increase in the abundance of proteins from the respiratory chain and oxidative phosphorylation system. In contrast, chronic epileptic tissue shows a 25-40% decrease in ATP production capacity. In summary, our study reveals that epilepsy leads to dynamic metabolic changes. Acute epileptic activity boosts ATP production, while chronic epilepsy reduces it significantly. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:58:08.521.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Loroch |
SpeciesList | scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-22 14:03:58 | ID requested | |
1 | 2024-09-12 07:53:27 | announced | |
⏵ 2 | 2024-10-22 06:58:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: epilepsy |
metabolic adaptation |
status epilepticus |
ATP production |
shotgun proteomics |
Contact List
Stefan Loroch |
contact affiliation | [1] QC-MS / Fa. Dr. Loroch, BioMedizinZentrum, Otto-Hahn-Straße 15, 44149 Dortmund [2] Medizinisches Proteom-Center, Center for Protein Diagnostics (PRODI), Medical Faculty, Ruhr-University Bochum, Bochum, Germany |
contact email | stefan@loroch.email |
lab head | |
Stefan Loroch |
contact affiliation | Medizinisches Proteome-Center der Ruhr Universität Bochum |
contact email | stefan@loroch.email |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055090
- Label: PRIDE project
- Name: Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment