PXD055066

PXD055066 is an original dataset announced via ProteomeXchange.

Title	Comparative protein profiling of stretched (low) and non-stretched podocytes
Description	Podocyte detachment due to mechanical stress is a common issue in hypertension-induced kidney disease. The present study investigated the proteome alterations in mechanically stretched podocytes, a model for glomerular hypertension in comparison to non-stretched cells. Conditionally immortalized differentiated podocytes were seeded on flexible silicon membranes of a six well plate, which was mounted on a manifold connected to a custom-built stretch apparatus (NIPOKA GmbH, Greifswald, Germany), which induced cyclic pressure variations resulting in upward and downward motion of the silicone membranes. Pressure amplitude was chosen to give a maximum up- and downward deflection of the membrane centre of 6 mm (low stretch).
HostingRepository	PRIDE
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-05-14_06:33:10.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elke Hammer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 06:13:00	ID requested
1	2025-05-13 07:42:00	announced
⏵ 2	2025-05-14 06:33:10	announced	2025-05-14: Updated project metadata.

10.1681/ASN.0000000706;

submitter keyword: Podocytes, mechanical stretch, LC-MS/MS

Elke Hammer
contact affiliation	Universitätsmedizin Greifswald Interfakultäres Institut für Genetik und Funktionelle Genomforschung Abteilung für Funktionelle Genomforschung Felix-Hausdorff-Straße 8 17487 Greifswald
contact email	hammer@uni-greifswald.de
lab head
Elke Hammer
contact affiliation	Dept. of Functional Genomics
contact email	hammer@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055066

PRIDE project URI

• PRIDE
  1. PXD055066
     1. Label: PRIDE project
     2. Name: Comparative protein profiling of stretched (low) and non-stretched podocytes