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PXD055065

PXD055065 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVault RNAs aid RNA virus infection by facilitating cytoplasmic localization of hnRNP C and ELAVL1
DescriptionAbstract Vault RNAs (vtRNAs) are a family of four small non-coding RNAs (ncRNAs) that are ubiquitously expressed in many eukaryotes and that regulate multiple cellular pathways. Their expression is increased upon infection with various DNA and RNA viruses. This suggests they either function to aid viral replication or as an antiviral restriction factor. However, their precise molecular function remains unclear. Here, we show that replication of picornaviruses, alphaviruses, and betacoronaviruses broadly enhances vtRNA expression. We find that genetic loss of vtRNAs inhibits replication of Sindbis virus (SINV) and encephalomyocarditis virus (EMCV) replication, independent of the antiviral type I interferon (IFN) response. A proteomic screen uncovered the vtRNA interactome and revealed that vtRNAs associate with RNA binding proteins ELAVL1 and hnRNP C in uninfected and infected cells. VtRNAs facilitate the translocation of ELAVL1 and hnRNP C from the nucleus to the cytoplasm in infected cells, an event that is required for efficient viral replication. Moreover, in the absence of vtRNAs, hnRNP C and ELAVL1 fail to associate with viral RNA in the cytosol of SINV-infected cells. Together, our findings uncover a novel molecular mechanism by which vtRNAs exert pro-viral activity during the course of SINV and EMCV infection, which opens up new avenues for therapeutic targeting to fight infectious diseases.
HostingRepositoryPRIDE
AnnounceDate2025-05-20
AnnouncementXMLSubmission_2025-05-20_01:46:29.534.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRayman Tjokrodirijo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-22 06:12:32ID requested
12025-05-20 01:46:29announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: host-pathogen interaction, small non-coding RNA, antiviral defense, RNA-binding proteins
Contact List
Peter A.
contact affiliationHead Proteomics Group, Leiden University Medical Center, Center for Proteomics and Metabolomics, PO Box 9600, Postal zone P1-Q, 2300 RC Leiden, The Netherlands
contact emailP.A.van_Veelen@lumc.nl
lab head
Rayman Tjokrodirijo
contact affiliationLeiden University Medical Center, Proteomics group
contact emailR.T.N.Tjokrodirijo@lumc.nl
dataset submitter
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