PXD055039

PXD055039 is an original dataset announced via ProteomeXchange.

Title	Evaluation of the embryogenic response through 6-benzyladenine in Coffea canephora in primed explants with auxin (1-naphthaleneacetic acid) plus cytokinin (kinetin) and protein profile.
Description	Plant somatic embryogenesis encompasses somatic cells switch into embryogenic cells that can later produce somatic embryos with the ability to produce plantlets. Using defined in vitro culture settings for the somatic embryogenesis process of Coffea canephora that consist of an adequation of plantlets with auxin plus cytokinin followed by cut-leaf explant cultivation with cytokinin, producing embryos with the ability to regenerate plantlets, the protein profile was explored in primed and control explants. We confirmed that cultivating cut-leaf explants with cytokinin is sufficient to promote the proliferation of somatic embryos and the high yield of somatic embryos in the protocol requires the adequation of plantlets with auxin plus cytokinin. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels reveal auxin- plus cytokinin-dependent regulated proteins in plantlets with up and down abundance. Chitinase A class III, proteins involved in the metabolism and folding of proteins, photosynthesis, antioxidant activity, and chromatin organization were identified. The RPN12 protein, which is a subunit of the proteasome 26S, has an abundance that is not associated with transcript changes, suggesting post-translational regulation.
HostingRepository	PRIDE
AnnounceDate	2024-10-02
AnnouncementXML	Submission_2024-10-02_12:19:40.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Aguilar-Hernandez
SpeciesList	scientific name: Coffea canephora; NCBI TaxID: 49390;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-22 06:04:08	ID requested
⏵ 1	2024-10-02 12:19:40	announced

Dataset with its publication pending

submitter keyword: 2D SDS-PAGE,LTQ-Orbitrap, auxin plud cytokinin, embryogenic response, Coffea canephora

Victor Aguilar-Hernández
contact affiliation	Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Calle 43, No. 130 Colonia Chuburná de Hidalgo, Mérida CP 97200, Yucatán, México.
contact email	victor.aguilar@cicy.mx
lab head
Victor Aguilar-Hernandez
contact affiliation	Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
contact email	victor.aguilar@cicy.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD055039

PRIDE project URI

• PRIDE
  1. PXD055039
     1. Label: PRIDE project
     2. Name: Evaluation of the embryogenic response through 6-benzyladenine in Coffea canephora in primed explants with auxin (1-naphthaleneacetic acid) plus cytokinin (kinetin) and protein profile.