PXD055023
PXD055023 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of Equine Platelet Lysate for Nebulization in the Horse |
| Description | Platelet lysate (PL) demonstrates antimicrobial and anti-inflammatory properties and may offer potential for treatment of bacterial pneumonia in horses. It remains unknown whether nebulization represents a suitable method of administration of PL in horses. This study characterized particle size and flow rate of pooled equine PL nebulized using an equine-specific nebulizer (Flexivent). Protein composition and antimicrobial activity were compared before and after nebulization. Protein composition was evaluated according to growth factor, antimicrobial peptide and cytokine concentrations and proteomic analysis. To evaluate antimicrobial activity, bacterial growth inhibition [maximum growth (µmax); carrying capacity (K)] were determined for E.coli, Streptococcus equi subsp zooepidemicus and Rhodococcus equi (WT and MDR) using pre- and post-nebulized PL concentrations of 50%. Flow rate and median particle size were 0.8 ml/min and 4.991 µm with 52% of particles ≤ 5 µm. Cytokine, growth factor, and antimicrobial peptide concentrations and proteomic profiles were similar pre- and post-nebulization. For all bacteria, nebulization did not alter effect of PL on growth parameters. Negative effects of PL treatments on K were noted for S. zooepidemicus (p= 0.009 all) compared to BHI. No effect of PL was observed on µmax for S.zooepidemicus and on K and µmax for E.coli. For R. equi (WT and MDR), K and µ were positively affected by PL treatments (p≤ 0.05). We concluded that nebulization does not appear to meaningfully impact PL bacterial growth inhibition or protein composition. Further research is recommended to better characterize PL antimicrobial activity against clinically relevant pathogens and suitability for nebulization in horses. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-12-26 |
| AnnouncementXML | Submission_2024-12-26_08:24:26.411.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055023 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Justine Arrington |
| SpeciesList | scientific name: Equus caballus; NCBI TaxID: 9796; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; deamidated L-glutamine; deamidated L-asparagine |
| Instrument | Orbitrap Fusion ETD; Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-08-20 11:59:48 | ID requested | |
| ⏵ 1 | 2024-12-26 08:24:26 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Platelet lysate, horse, nebulization, respiratory, antimicrobial |
Contact List
| Kara Lascola | |
|---|---|
| lab head | |
| Justine Arrington | |
| contact affiliation | University of Illinois at Urbana-Champaign |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003292/ |
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