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PXD055019

PXD055019 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive analysis the effect of arsenic exposure on the virulence and antibiotic resistance of Pseudomonas aeruginosa
DescriptionNatural or industrial pollution of source water by arsenic poses a serious threat to the health of tens of millions of people worldwide, while limited data have addressed its influence on the waterborne pathogens. This study examines the effects of arsenic exposure on Pseudomonas aeruginosa, an opportunistic pathogen that poses a health hazard in public water supplies. Our findings reveal that arsenic exposure at concentrations of 0.12–20 mg/L As(III) causes rapid growth arrest in P. aeruginosa. This exposure also leads to a marked decrease in elastase, pyocyanin, and flagellar production, while significantly increasing siderophore and extracellular polysaccharide production. Proteomic analysis indicates that both low (0.12 mg/L) and high (1.2 mg/L) arsenic levels trigger an antioxidant defense response, evidenced by the upregulation of Fnr-2, TrxB2, Ohr, AlgZ, and PslG. Concurrently, proteins related to basal metabolism, quorum sensing, T3SS, pyocyanin biosynthesis, and flagellar assembly are downregulated. Moreover, arsenic exposure enhances the expression of proteins involved in siderophore biosynthesis and transporters associated with iron uptake and multidrug resistance. In vitro assays further demonstrate that arsenic exposure reduces bacterial cytotoxicity while promoting survival and proliferation under antibiotic treatment. In summary, this study provides comprehensive insights into how arsenic affects bacterial virulence and antibiotic tolerance in P. aeruginosa, highlighting the potential public health risks posed by the frequent co-occurrence of arsenic-contaminated water sources and multidrug-resistant bacteria.
HostingRepositoryiProX
AnnounceDate2024-08-20
AnnouncementXMLSubmission_2024-08-20_03:31:51.577.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZe Li
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListNo PTMs are included in the dataset
InstrumentThermo Fisher Scientific instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-20 03:12:29ID requested
12024-08-20 03:31:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Source water, Arsenic exposure, P. aeruginosa, Bacterial virulence, Antibiotic tolerance
Contact List
Yingjie Song
contact affiliationCollege of Life Science, Sichuan Normal University
contact email1127551970@qq.com
lab head
Ze Li
contact affiliationSichuan University
contact email1477854366@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI