PXD055019

PXD055019 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis the effect of arsenic exposure on the virulence and antibiotic resistance of Pseudomonas aeruginosa
Description	Natural or industrial pollution of source water by arsenic poses a serious threat to the health of tens of millions of people worldwide, while limited data have addressed its influence on the waterborne pathogens. This study examines the effects of arsenic exposure on Pseudomonas aeruginosa, an opportunistic pathogen that poses a health hazard in public water supplies. Our findings reveal that arsenic exposure at concentrations of 0.12–20 mg/L As(III) causes rapid growth arrest in P. aeruginosa. This exposure also leads to a marked decrease in elastase, pyocyanin, and flagellar production, while significantly increasing siderophore and extracellular polysaccharide production. Proteomic analysis indicates that both low (0.12 mg/L) and high (1.2 mg/L) arsenic levels trigger an antioxidant defense response, evidenced by the upregulation of Fnr-2, TrxB2, Ohr, AlgZ, and PslG. Concurrently, proteins related to basal metabolism, quorum sensing, T3SS, pyocyanin biosynthesis, and flagellar assembly are downregulated. Moreover, arsenic exposure enhances the expression of proteins involved in siderophore biosynthesis and transporters associated with iron uptake and multidrug resistance. In vitro assays further demonstrate that arsenic exposure reduces bacterial cytotoxicity while promoting survival and proliferation under antibiotic treatment. In summary, this study provides comprehensive insights into how arsenic affects bacterial virulence and antibiotic tolerance in P. aeruginosa, highlighting the potential public health risks posed by the frequent co-occurrence of arsenic-contaminated water sources and multidrug-resistant bacteria.
HostingRepository	iProX
AnnounceDate	2024-08-20
AnnouncementXML	Submission_2024-08-20_03:31:51.577.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ze Li
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-20 03:12:29	ID requested
⏵ 1	2024-08-20 03:31:52	announced

Dataset with its publication pending

submitter keyword: Source water, Arsenic exposure, P. aeruginosa, Bacterial virulence, Antibiotic tolerance

Yingjie Song
contact affiliation	College of Life Science, Sichuan Normal University
contact email	1127551970@qq.com
lab head
Ze Li
contact affiliation	Sichuan University
contact email	1477854366@qq.com
dataset submitter

iProX dataset URI