PXD055008 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans |
Description | Antimicrobial-induced DNA damage, and subsequent repair via upregulation of DNA repair factors, including error-prone translesion polymerases, can lead to the increased accumulation of mutations in the microbial genome, and ultimately increased risk of acquired mutations associated with antimicrobial resistance. While this phenotype is well described in bacterial species, it is less thoroughly investigated amongst microbial fungi. Here, we monitor DNA damage induced by antifungal agents in the fungal pathogen Candida albicans, and find that commonly used antifungal drugs are able to induce DNA damage, leading to the upregulation of transcripts encoding predicted error-prone polymerases and related factors. We focus on REV1, encoding a putative error-prone polymerase, and find that while deleting this gene in C. albicans leads to increased sensitivity to DNA damage, it also unexpectedly renders cells more likely to incur mutations and evolve resistance to antifungal agents. We further find that deletion of REV1 leads to a significant depletion in the uncharacterized protein Shm1, which itself plays a role in fungal mutagenesis. Together, this work lends new insight into previously uncharacterized factors with important roles in the DNA damage response, mutagenesis, and the evolution of antifungal drug resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_00:32:57.868.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD055008 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rebecca Shapiro |
SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-19 14:57:18 | ID requested | |
⏵ 1 | 2025-05-07 00:32:58 | announced | |
Publication List
10.6019/PXD055008; |
Agyare-Tabbi MR, Uthayakumar D, Francis D, Maroc L, Grant C, McQueen P, Westmacott G, Shaker H, Skulska I, Gagnon-Arsenault I, Boisvert J, Landry CR, Shapiro RS, . NPJ Antimicrob Resist, 2(1):42(2024) [pubmed] |
10.1038/s44259-024-00057-0; |
Keyword List
submitter keyword: Candida albicans, REV1, fungal |
Contact List
Rebecca Shapiro |
contact affiliation | Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
contact email | shapiror@uoguelph.ca |
lab head | |
Rebecca Shapiro |
contact affiliation | University of Guelph |
contact email | shapiror@uoguelph.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD055008 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD055008
- Label: PRIDE project
- Name: A role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans