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PXD055008

PXD055008 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans
DescriptionAntimicrobial-induced DNA damage, and subsequent repair via upregulation of DNA repair factors, including error-prone translesion polymerases, can lead to the increased accumulation of mutations in the microbial genome, and ultimately increased risk of acquired mutations associated with antimicrobial resistance. While this phenotype is well described in bacterial species, it is less thoroughly investigated amongst microbial fungi. Here, we monitor DNA damage induced by antifungal agents in the fungal pathogen Candida albicans, and find that commonly used antifungal drugs are able to induce DNA damage, leading to the upregulation of transcripts encoding predicted error-prone polymerases and related factors. We focus on REV1, encoding a putative error-prone polymerase, and find that while deleting this gene in C. albicans leads to increased sensitivity to DNA damage, it also unexpectedly renders cells more likely to incur mutations and evolve resistance to antifungal agents. We further find that deletion of REV1 leads to a significant depletion in the uncharacterized protein Shm1, which itself plays a role in fungal mutagenesis. Together, this work lends new insight into previously uncharacterized factors with important roles in the DNA damage response, mutagenesis, and the evolution of antifungal drug resistance.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_00:32:57.868.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD055008
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRebecca Shapiro
SpeciesList scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-19 14:57:18ID requested
12025-05-07 00:32:58announced
Publication List
10.6019/PXD055008;
Agyare-Tabbi MR, Uthayakumar D, Francis D, Maroc L, Grant C, McQueen P, Westmacott G, Shaker H, Skulska I, Gagnon-Arsenault I, Boisvert J, Landry CR, Shapiro RS, . NPJ Antimicrob Resist, 2(1):42(2024) [pubmed]
10.1038/s44259-024-00057-0;
Keyword List
submitter keyword: Candida albicans, REV1, fungal
Contact List
Rebecca Shapiro
contact affiliationDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
contact emailshapiror@uoguelph.ca
lab head
Rebecca Shapiro
contact affiliationUniversity of Guelph
contact emailshapiror@uoguelph.ca
dataset submitter
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Dataset FTP location
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