PXD055008

PXD055008 is an original dataset announced via ProteomeXchange.

Title	A role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans
Description	Antimicrobial-induced DNA damage, and subsequent repair via upregulation of DNA repair factors, including error-prone translesion polymerases, can lead to the increased accumulation of mutations in the microbial genome, and ultimately increased risk of acquired mutations associated with antimicrobial resistance. While this phenotype is well described in bacterial species, it is less thoroughly investigated amongst microbial fungi. Here, we monitor DNA damage induced by antifungal agents in the fungal pathogen Candida albicans, and find that commonly used antifungal drugs are able to induce DNA damage, leading to the upregulation of transcripts encoding predicted error-prone polymerases and related factors. We focus on REV1, encoding a putative error-prone polymerase, and find that while deleting this gene in C. albicans leads to increased sensitivity to DNA damage, it also unexpectedly renders cells more likely to incur mutations and evolve resistance to antifungal agents. We further find that deletion of REV1 leads to a significant depletion in the uncharacterized protein Shm1, which itself plays a role in fungal mutagenesis. Together, this work lends new insight into previously uncharacterized factors with important roles in the DNA damage response, mutagenesis, and the evolution of antifungal drug resistance.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_00:32:57.868.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD055008
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebecca Shapiro
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-19 14:57:18	ID requested
⏵ 1	2025-05-07 00:32:58	announced

10.6019/PXD055008;

Agyare-Tabbi MR, Uthayakumar D, Francis D, Maroc L, Grant C, McQueen P, Westmacott G, Shaker H, Skulska I, Gagnon-Arsenault I, Boisvert J, Landry CR, Shapiro RS, . NPJ Antimicrob Resist, 2(1):42(2024) [pubmed]

10.1038/s44259-024-00057-0;

submitter keyword: Candida albicans, REV1, fungal

Rebecca Shapiro
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada
contact email	shapiror@uoguelph.ca
lab head
Rebecca Shapiro
contact affiliation	University of Guelph
contact email	shapiror@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055008
     1. Label: PRIDE project
     2. Name: A role for the putative error-prone polymerase REV1 in DNA damage and antifungal drug resistance in Candida albicans