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PXD054994

PXD054994 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment
DescriptionIn this study, we employed bio-orthogonal non-canonical amino acid tagging (BONCAT) combined with iTRAQ-based quantitative proteomics to investigate the effects of pyrimethamine (PM) on newly synthesised proteins (NSPs) in Toxoplasma gondii. Parasites were treated with varying concentrations of PM, followed by mass spectrometric analysis to identify and quantify NSPs. We observed a concentration-dependent response, where several NSPs were downregulated at low PM doses but upregulated at higher concentrations. Notably, ribosomal proteins, invasion-related proteins (e.g., ROP26), and stress response proteins (e.g., serine/threonine-protein phosphatase 2B) showed significant changes in expression. The upregulation of calcineurin-related proteins suggests an activation of calcium-dependent stress response pathways, possibly as a compensatory mechanism to counteract the drug’s inhibitory effects. Our findings reveal the dynamic regulatory mechanisms of T. gondii under PM treatment, highlighting the parasite’s adaptive strategies to survive drug-induced stress. This study underscores the utility of BONCAT-iTRAQ proteomics in uncovering the molecular responses of pathogens to therapeutic interventions, providing insights that could inform the development of more effective anti-parasitic strategies.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_00:32:07.806.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarunakaran Kalesh
SpeciesList scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-19 05:31:07ID requested
12025-05-07 00:32:08announced
Publication List
10.3390/pathogens13100879;
Mina JG, Parthasarathy A, Porta EO, Denny PW, Kalesh K, to Pyrimethamine Treatment. Pathogens, 13(10):(2024) [pubmed]
Keyword List
submitter keyword: BONCAT, pyrimethamine,Toxoplasma gondii, quantitative proteomics, iTRAQ
Contact List
Kalesh Karunakaran
contact affiliationSchool of Health & Life Sciences, Teesside University, UK
contact emailk.karunakaran@tees.ac.uk
lab head
Karunakaran Kalesh
contact affiliationTeesside University
contact emailk.karunakaran@tees.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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