PXD054994
PXD054994 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment |
Description | In this study, we employed bio-orthogonal non-canonical amino acid tagging (BONCAT) combined with iTRAQ-based quantitative proteomics to investigate the effects of pyrimethamine (PM) on newly synthesised proteins (NSPs) in Toxoplasma gondii. Parasites were treated with varying concentrations of PM, followed by mass spectrometric analysis to identify and quantify NSPs. We observed a concentration-dependent response, where several NSPs were downregulated at low PM doses but upregulated at higher concentrations. Notably, ribosomal proteins, invasion-related proteins (e.g., ROP26), and stress response proteins (e.g., serine/threonine-protein phosphatase 2B) showed significant changes in expression. The upregulation of calcineurin-related proteins suggests an activation of calcium-dependent stress response pathways, possibly as a compensatory mechanism to counteract the drug’s inhibitory effects. Our findings reveal the dynamic regulatory mechanisms of T. gondii under PM treatment, highlighting the parasite’s adaptive strategies to survive drug-induced stress. This study underscores the utility of BONCAT-iTRAQ proteomics in uncovering the molecular responses of pathogens to therapeutic interventions, providing insights that could inform the development of more effective anti-parasitic strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_00:32:07.806.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Karunakaran Kalesh |
SpeciesList | scientific name: Toxoplasma gondii; NCBI TaxID: 5811; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-08-19 05:31:07 | ID requested | |
⏵ 1 | 2025-05-07 00:32:08 | announced |
Publication List
10.3390/pathogens13100879; |
Mina JG, Parthasarathy A, Porta EO, Denny PW, Kalesh K, to Pyrimethamine Treatment. Pathogens, 13(10):(2024) [pubmed] |
Keyword List
submitter keyword: BONCAT, pyrimethamine,Toxoplasma gondii, quantitative proteomics, iTRAQ |
Contact List
Kalesh Karunakaran | |
---|---|
contact affiliation | School of Health & Life Sciences, Teesside University, UK |
contact email | k.karunakaran@tees.ac.uk |
lab head | |
Karunakaran Kalesh | |
contact affiliation | Teesside University |
contact email | k.karunakaran@tees.ac.uk |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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