PXD054994

PXD054994 is an original dataset announced via ProteomeXchange.

Title	Molecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment
Description	In this study, we employed bio-orthogonal non-canonical amino acid tagging (BONCAT) combined with iTRAQ-based quantitative proteomics to investigate the effects of pyrimethamine (PM) on newly synthesised proteins (NSPs) in Toxoplasma gondii. Parasites were treated with varying concentrations of PM, followed by mass spectrometric analysis to identify and quantify NSPs. We observed a concentration-dependent response, where several NSPs were downregulated at low PM doses but upregulated at higher concentrations. Notably, ribosomal proteins, invasion-related proteins (e.g., ROP26), and stress response proteins (e.g., serine/threonine-protein phosphatase 2B) showed significant changes in expression. The upregulation of calcineurin-related proteins suggests an activation of calcium-dependent stress response pathways, possibly as a compensatory mechanism to counteract the drug’s inhibitory effects. Our findings reveal the dynamic regulatory mechanisms of T. gondii under PM treatment, highlighting the parasite’s adaptive strategies to survive drug-induced stress. This study underscores the utility of BONCAT-iTRAQ proteomics in uncovering the molecular responses of pathogens to therapeutic interventions, providing insights that could inform the development of more effective anti-parasitic strategies.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_00:32:07.806.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karunakaran Kalesh
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-19 05:31:07	ID requested
⏵ 1	2025-05-07 00:32:08	announced

10.3390/pathogens13100879;

Mina JG, Parthasarathy A, Porta EO, Denny PW, Kalesh K, to Pyrimethamine Treatment. Pathogens, 13(10):(2024) [pubmed]

submitter keyword: BONCAT, pyrimethamine,Toxoplasma gondii, quantitative proteomics, iTRAQ

Kalesh Karunakaran
contact affiliation	School of Health & Life Sciences, Teesside University, UK
contact email	k.karunakaran@tees.ac.uk
lab head
Karunakaran Kalesh
contact affiliation	Teesside University
contact email	k.karunakaran@tees.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054994

PRIDE project URI

• PRIDE
  1. PXD054994
     1. Label: PRIDE project
     2. Name: Molecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment