PXD054974

PXD054974 is an original dataset announced via ProteomeXchange.

Title	Evidence for substrate control of Cdk phosphorylation timing during the budding yeast cell cycle 2522
Description	The main intention of this project is to investigate how Cdk substrate phosphorylation is ordered during the cell cycle. We want to understand what makes a ‘good’ Cdk substrate that is phosphorylated early during the cell cycle, e. g. to promote S phase, as compared to a ‘bad’ Cdk substrate that is phosphorylated much later, e. g. when cells enter mitosis. This could be because a late substrate is indeed a poor Cdk substrate, or it could be that the late substrate is a good substrate for Cdk counteracting phosphatases. The two possibilities are not mutually exclusive. We want to compare unperturbed phosphorylation and rephosphorylation from an artificially low phosphorylation state in metaphase arrest. To do that, we arrest cells in metaphase, inhibit Cdk until substrates get dephosphorylated, and reactivate Cdk and record the time and kinetics of the substrate rephosphorylation.
HostingRepository	PRIDE
AnnounceDate	2025-04-10
AnnouncementXML	Submission_2025-04-10_01:57:31.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Flynn
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-17 10:14:54	ID requested
⏵ 1	2025-04-10 01:57:32	announced

10.1016/J.CELREP.2025.115534;

submitter keyword: Phosphoproteomics, TMT,Cdk, Cell cycle

Frank Uhlmann
contact affiliation	Chromosome Segregation Laboratory The Francis Crick Institute Midland Road London NW1 1AT
contact email	frank.uhlmann@crick.ac.uk
lab head
Helen Flynn
contact affiliation	The Francis Crick Institute
contact email	helen.flynn@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054974

PRIDE project URI

• PRIDE
  1. PXD054974
     1. Label: PRIDE project
     2. Name: Evidence for substrate control of Cdk phosphorylation timing during the budding yeast cell cycle 2522