PXD054972

PXD054972 is an original dataset announced via ProteomeXchange.

Title	Structural basis for membrane remodelling by the AP5:SPG11-SPG15 complex
Description	The human spastizin (spastic paraplegia 15, SPG15) and spatacsin (spastic paraplegia 11, SPG11) complex is involved in the formation of lysosomes, and mutations in these two proteins are linked with hereditary autosomal recessive spastic paraplegia (HSP). SPG11-SPG15 can cooperate with the fifth adaptor protein complex (AP5) involved in membrane sorting of late endosomes. We employed cryo-electron microscopy and in silico predictions to investigate the structural assemblies of SPG11-SPG15 and AP5:SPG11-SPG15 complex. The W-shaped SPG11-SPG15 intertwined in a head-to-head fashion, and the N-terminal region of SPG11 is required for AP5 complex interaction and assembly. The AP5 complex is in a super open conformation. Our findings reveal that the AP5:SPG11-SPG15 complex can bind PI3P molecules, sense membrane curvature and drive membrane remodelling in vitro. These studies provide key insights into the structure and function of the spastic paraplegia AP5:SPG11-SPG15 complex, which is essential for the initiation of autolysosome tubulation.
HostingRepository	PRIDE
AnnounceDate	2025-03-20
AnnouncementXML	Submission_2025-03-20_02:28:41.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Goran Stjepanovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-17 06:16:45	ID requested
⏵ 1	2025-03-20 02:28:43	announced

10.1038/S41594-025-01500-0;

submitter keyword: XL-MS, LC-MS

Goran Stjepanovic
contact affiliation	Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen, Shenzhen 518172, China.
contact email	goranstjepanovic@cuhk.edu.cn
lab head
Goran Stjepanovic
contact affiliation	CUHKSZ
contact email	goranstjepanovic@cuhk.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054972

PRIDE project URI

• PRIDE
  1. PXD054972
     1. Label: PRIDE project
     2. Name: Structural basis for membrane remodelling by the AP5:SPG11-SPG15 complex