PXD054968

PXD054968 is an original dataset announced via ProteomeXchange.

Title	Lysosomal proteome characterization during TRPML1 activation by MLSA5 in human cancer cells
Description	To capture the changes in the lysosomal proteome upon TRPML1 activation, achieved using a small molecule agonist MLSA5, human cancer cells, with or without gene-editing, stably expressing TMEM192-mRFP-3xHA (Lyso-IP), were treated with MLSA5 and Lyso-IP samples were prepared and processed for TMT-MS analysis. Each condition was performed in triplicate.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_10:30:54.493.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054968
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chan Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-16 16:04:20	ID requested
⏵ 1	2025-04-22 10:30:54	announced

10.6019/PXD054968;

submitter keyword: MLSA5, TMT, A101D, V-ATPase,Lysosome, TRPML1, DMXL2, DMXL1, Lyso-IP, U2OS, ATG16L1

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	wade_harper@hms.harvard.edu
lab head
Chan Lee
contact affiliation	Harvard Medical School
contact email	chan_lee@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054968

PRIDE project URI

• PRIDE
  1. PXD054968
     1. Label: PRIDE project
     2. Name: Lysosomal proteome characterization during TRPML1 activation by MLSA5 in human cancer cells