PXD054967

PXD054967 is an original dataset announced via ProteomeXchange.

Title	DMXL1 interaction proteomics from U2OS cells
Description	To identify protein interactions with DMXL1, U2OS cells were engineered to either stably over-express DMXL1 tagged with mNeonGreen (mNG) or express HA-dTAG-tagged DMXL1 from the endogenous locus. Tagged DMXL1 was pulled-down by immunoprecipitation (IP) and IP samples were processed for TMT-MS analysis. As negative control, pull-down was also performed from parental U2OS cells. Each condition was performed in triplicate.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_10:26:36.086.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chan Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-16 15:42:55	ID requested
⏵ 1	2025-04-22 10:26:37	announced

10.6019/PXD054967;

submitter keyword: Lysosome,DMXL1, V-ATPase, DMXL2, U2OS

J. Wade
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	wade_harper@hms.harvard.edu
lab head
Chan Lee
contact affiliation	Harvard Medical School
contact email	chan_lee@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054967

PRIDE project URI

• PRIDE
  1. PXD054967
     1. Label: PRIDE project
     2. Name: DMXL1 interaction proteomics from U2OS cells